Chapter title |
Hedgehog Signaling Protocols
|
---|---|
Chapter number | 6 |
Book title |
Hedgehog Signaling Protocols
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2772-2_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2771-5, 978-1-4939-2772-2
|
Authors |
Fu, Lin, Lv, Xiangdong, Xiong, Yue, Zhao, Yun, Lin Fu, Xiangdong Lv, Yue Xiong, Yun Zhao |
Abstract |
Protein-protein interactions and signal-induced protein conformational changes are fundamental molecular events that are considered as essential in modern life sciences. Among various techniques developed to study such phenomena, fluorescence resonance energy transfer (FRET) is a widely used method with many advantages in detecting these molecular events. Here, we describe the application of FRET in the mechanistic investigation of cell signal transduction, taking the example of the Hh signaling pathway, which plays a critical role in embryonic development and tissue homeostasis. A number of general guidelines as well as some key notes have been summarized as a protocol for reader's reference. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 1 | 33% |
Unknown | 2 | 67% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Unknown | 2 | 67% |