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Glaucoma

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Cover of 'Glaucoma'

Table of Contents

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    Book Overview
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    Chapter 1 Use of Animal Models and Techniques in Glaucoma Research: Introduction
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    Chapter 2 Hypertonic Saline Injection Model of Experimental Glaucoma in Rats
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    Chapter 3 The Microbead Occlusion Model of Ocular Hypertension in Mice
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    Chapter 4 Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods
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    Chapter 5 Noninvasive Intraocular Pressure Measurement in Animals Models of Glaucoma
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    Chapter 6 High-Throughput Binocular Pattern Electroretinograms in the Mouse
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    Chapter 7 Visual Evoked Potentials in Glaucoma and Alzheimer’s Disease
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    Chapter 8 Investigation of the Functional Retinal Output Using Microelectrode Arrays
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    Chapter 9 Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling
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    Chapter 10 Shotgun Sphingolipid Analysis of Human Aqueous Humor
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    Chapter 11 Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion
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    Chapter 12 Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models
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    Chapter 13 Quantification of Scleral Biomechanics and Collagen Fiber Alignment
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    Chapter 14 Biolistic Labeling of Retinal Ganglion Cells
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    Chapter 15 Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma
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    Chapter 16 In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration
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    Chapter 17 3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues
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    Chapter 18 Visualizing Astrocytes of the Optic Nerve
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    Chapter 19 Investigation of MicroRNA Expression in Experimental Glaucoma
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    Chapter 20 Utilizing RNA-Seq to Identify Differentially Expressed Genes in Glaucoma Model Tissues, Such as the Rodent Optic Nerve Head
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    Chapter 21 Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve
Attention for Chapter 4: Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods
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Chapter title
Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods
Chapter number 4
Book title
Glaucoma
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7407-8_4
Pubmed ID
Book ISBNs
978-1-4939-7406-1, 978-1-4939-7407-8
Authors

Elena Vecino, Haritz Urcola, Alejando Bayon, Sansar C. Sharma

Abstract

Two methods to induce elevation of the intraocular pressure (experimental glaucoma) are described in the present chapter. The first method is based on increasing the post-trabecular resistance to aqueous outflow by cauterizing the episcleral veins (EVC). This method allows the observation of ultrastructural changes in the trabecular meshwork (TM) without interfering with any structure within the eye such as TM, ciliary body, and/or the Retina. The second method is the multiple injection of microbeads into the anterior chamber, as a pre and intra-trabecular method that induce secondary effects on the TM cells. Both methods lead to an increase in IOP.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 22%
Student > Doctoral Student 1 11%
Librarian 1 11%
Lecturer > Senior Lecturer 1 11%
Unspecified 1 11%
Other 2 22%
Unknown 1 11%
Readers by discipline Count As %
Neuroscience 3 33%
Agricultural and Biological Sciences 1 11%
Biochemistry, Genetics and Molecular Biology 1 11%
Social Sciences 1 11%
Medicine and Dentistry 1 11%
Other 1 11%
Unknown 1 11%