| Chapter title |
Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling
|
|---|---|
| Chapter number | 9 |
| Book title |
Glaucoma
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7407-8_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7406-1, 978-1-4939-7407-8
|
| Authors |
Anna Trzeciecka, Padmanabhan Pattabiraman, Maria Carmen Piqueras, Carol Toris, Sanjoy K. Bhattacharya, Trzeciecka, Anna, Pattabiraman, Padmanabhan, Piqueras, Maria Carmen, Toris, Carol, Bhattacharya, Sanjoy K. |
| Abstract |
iTRAQ 4plex method enables multiplexing of up to four samples for simultaneous quantitation to improve sensitivity and scope of proteomic analysis. Here, we describe iTRAQ 4plex labeling of human aqueous humor specimens followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and data analysis for peptide identification and quantitation using Proteome Discoverer software. The protocol can be applied for other animals as well; however, pooling of specimens may be required to obtain sufficient amount of protein for labeling. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 69 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 2 | 3% |
| Professor | 1 | 1% |
| Student > Ph. D. Student | 1 | 1% |
| Researcher | 1 | 1% |
| Unknown | 64 | 93% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 2 | 3% |
| Biochemistry, Genetics and Molecular Biology | 1 | 1% |
| Neuroscience | 1 | 1% |
| Medicine and Dentistry | 1 | 1% |
| Unknown | 64 | 93% |