↓ Skip to main content

Glaucoma

Overview of attention for book
Cover of 'Glaucoma'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Use of Animal Models and Techniques in Glaucoma Research: Introduction
  3. Altmetric Badge
    Chapter 2 Hypertonic Saline Injection Model of Experimental Glaucoma in Rats
  4. Altmetric Badge
    Chapter 3 The Microbead Occlusion Model of Ocular Hypertension in Mice
  5. Altmetric Badge
    Chapter 4 Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods
  6. Altmetric Badge
    Chapter 5 Noninvasive Intraocular Pressure Measurement in Animals Models of Glaucoma
  7. Altmetric Badge
    Chapter 6 High-Throughput Binocular Pattern Electroretinograms in the Mouse
  8. Altmetric Badge
    Chapter 7 Visual Evoked Potentials in Glaucoma and Alzheimer’s Disease
  9. Altmetric Badge
    Chapter 8 Investigation of the Functional Retinal Output Using Microelectrode Arrays
  10. Altmetric Badge
    Chapter 9 Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling
  11. Altmetric Badge
    Chapter 10 Shotgun Sphingolipid Analysis of Human Aqueous Humor
  12. Altmetric Badge
    Chapter 11 Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion
  13. Altmetric Badge
    Chapter 12 Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models
  14. Altmetric Badge
    Chapter 13 Quantification of Scleral Biomechanics and Collagen Fiber Alignment
  15. Altmetric Badge
    Chapter 14 Biolistic Labeling of Retinal Ganglion Cells
  16. Altmetric Badge
    Chapter 15 Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma
  17. Altmetric Badge
    Chapter 16 In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration
  18. Altmetric Badge
    Chapter 17 3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues
  19. Altmetric Badge
    Chapter 18 Visualizing Astrocytes of the Optic Nerve
  20. Altmetric Badge
    Chapter 19 Investigation of MicroRNA Expression in Experimental Glaucoma
  21. Altmetric Badge
    Chapter 20 Utilizing RNA-Seq to Identify Differentially Expressed Genes in Glaucoma Model Tissues, Such as the Rodent Optic Nerve Head
  22. Altmetric Badge
    Chapter 21 Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve
Attention for Chapter 2: Hypertonic Saline Injection Model of Experimental Glaucoma in Rats
Altmetric Badge

Citations

dimensions_citation
1 Dimensions

Readers on

mendeley
4 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Hypertonic Saline Injection Model of Experimental Glaucoma in Rats
Chapter number 2
Book title
Glaucoma
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7407-8_2
Pubmed ID
Book ISBNs
978-1-4939-7406-1, 978-1-4939-7407-8
Authors

John C. Morrison, Elaine C. Johnson, William O. Cepurna

Abstract

A reliable method of creating chronic elevation of intraocular pressure (IOP) in rodents is an important tool in reproducing and studying the mechanisms of optic nerve injury that occur in glaucoma. In addition, such a model could provide a valuable method for testing potential neuroprotective treatments. This paper outlines the basic methods for producing obstruction of aqueous humor outflow and IOP elevation by injecting hypertonic saline (a sclerosant) into the aqueous outflow pathway. This is one of several rodent glaucoma models in use today. In this method, a plastic ring is placed around the equator of the eye to restrict injected saline to the limbus. By inserting a small glass microneedle in an aqueous outflow vein in the episclera and injecting hypertonic saline toward the limbus, the saline is forced into Schlemm's canal and across the trabecular meshwork. The resultant inflammation and scarring of the anterior chamber angle occurs gradually, resulting in a rise in IOP after approximately 1 week. This article will describe the equipment necessary for producing this model and the steps of the technique itself.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 50%
Professor 1 25%
Student > Doctoral Student 1 25%
Readers by discipline Count As %
Neuroscience 2 50%
Medicine and Dentistry 1 25%
Agricultural and Biological Sciences 1 25%