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Gene Expression Profiling

Overview of attention for book
Cover of 'Gene Expression Profiling'

Table of Contents

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    Book Overview
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    Chapter 1 Real-Time PCR and Multiplex Approaches
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    Chapter 2 Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs
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    Chapter 3 Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals
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    Chapter 4 Software and Tools for Microarray Data Analysis
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    Chapter 5 Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumour Cells
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    Chapter 6 Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay
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    Chapter 7 MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs.
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    Chapter 8 Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases
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    Chapter 9 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins
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    Chapter 10 Gene Expression Profiling
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    Chapter 11 Immunohistochemical and Immunofluorescence Procedures for Protein Analysis
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    Chapter 12 Advanced Microscopy: Laser Scanning Confocal Microscopy
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    Chapter 13 Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling.
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    Chapter 14 Atomic Force Microscopy and High-Content Analysis: Two Innovative Technologies for Dissecting the Relationship Between Epithelial–Mesenchymal Transition-Related Morphological and Structural Alterations and Cell Mechanical Properties
Attention for Chapter 13: Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling.
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Chapter title
Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling.
Chapter number 13
Book title
Gene Expression Profiling
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-289-2_13
Pubmed ID
Book ISBNs
978-1-61779-288-5, 978-1-61779-289-2

Sweta Rani, Keith O’Brien, Fergal C. Kelleher, Claire Corcoran, Serena Germano, Marek W. Radomski, John Crown, Lorraine O’Driscoll


Exosomes are nano-sized, cell membrane surrounded structures that are released from many cell types. These exosomes are believed to transport a range of molecules, including mRNAs, miRNAs, and proteins; the contents depending on their cell of origin. The physiological and pathological relevance of exosomes has yet to be fully elucidated. Exosomes have been implicated in cell-to-cell communication. For example, in relation to the immune system, such exosomes may enable exchange of antigen or major histocompatibility complex-peptide complexes between antigen-bearing cells and antigen-presenting cells; in cancer, they may contain molecules that not only have relevance as biomarkers, but may also be taken up and cause adverse effects on secondary cells. Furthermore, exosomes have been proposed as autologous delivery systems that could be exploited for personalised delivery of therapeutics. In order to explore the contents and functional relevance of exosomes from medium conditioned by culture cells or from other biological fluids, prior to extensive molecular profiling, they must be isolated and purified. Here, we describe differential centrifugation methods suitable for isolating exosomes from conditioned medium and from other biological fluids, including serum, saliva, tumour ascites, and urine. We also detail Western blotting and transmission electron microscopy methods suitable for basic assessment of their presence, size, and purity, prior to progressing to global mRNA, miRNA, or protein profiling.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 97 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 4%
Germany 1 1%
Singapore 1 1%
Unknown 91 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 26 27%
Student > Ph. D. Student 24 25%
Student > Master 10 10%
Student > Doctoral Student 8 8%
Professor > Associate Professor 6 6%
Other 13 13%
Unknown 10 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 31%
Medicine and Dentistry 18 19%
Biochemistry, Genetics and Molecular Biology 16 16%
Chemistry 5 5%
Pharmacology, Toxicology and Pharmaceutical Science 3 3%
Other 11 11%
Unknown 14 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 September 2011.
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