Chapter title |
Grapevine (Vitis vinifera L.).
|
---|---|
Chapter number | 15 |
Book title |
Agrobacterium Protocols
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-1658-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1657-3, 978-1-4939-1658-0
|
Authors |
Laurent Torregrosa, Sandrine Vialet, Angélique Adivèze, Pat Iocco-Corena, Mark R Thomas, Mark R. Thomas, Torregrosa, Laurent, Vialet, Sandrine, Adivèze, Angélique, Iocco-Corena, Pat, Thomas, Mark R. |
Abstract |
Grapevine (Vitis) is considered to be one of the major fruit crops in the world based on hectares cultivated and economic value. Grapes are used not only for wine but also for fresh fruit, dried fruit, and juice production. Wine is by far the major product of grapes, and the focus of this chapter is on wine grape cultivars. Grapevine cultivars of Vitis vinifera L. have a reputation for producing premium quality wines. These premium quality wines are produced from a small number of cultivars that enjoy a high level of consumer acceptance and are firmly entrenched in the market place because of varietal name branding and the association of certain wine styles and regions with specific cultivars. In light of this situation, grapevine improvement by a transgenic approach is attractive when compared to a classical breeding approach. The transfer of individual traits as single genes with a minimum disruption to the original genome would leave the traditional characteristics of the cultivar intact. However, a reliable transformation system is required for a successful transgenic approach to grapevine improvement. There are three criteria for achieving an efficient Agrobacterium-mediated transformation system: (1) the production of highly regenerative transformable tissue, (2) optimal cocultivation conditions for both grapevine tissue and Agrobacterium, and (3) an efficient selection regime for transgenic plant regeneration. In this chapter, we describe a grapevine transformation system that meets these criteria. We also describe a protocol for the production of transformed roots suitable for functional gene studies and for the production of semi-transgenic grafted plants. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Germany | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 74 | 97% |
Demographic breakdown
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Researcher | 13 | 17% |
Student > Ph. D. Student | 11 | 14% |
Student > Doctoral Student | 7 | 9% |
Student > Master | 7 | 9% |
Student > Bachelor | 6 | 8% |
Other | 6 | 8% |
Unknown | 26 | 34% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 26 | 34% |
Biochemistry, Genetics and Molecular Biology | 7 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
Social Sciences | 2 | 3% |
Engineering | 2 | 3% |
Other | 5 | 7% |
Unknown | 32 | 42% |