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Agrobacterium Protocols

Overview of attention for book
Cover of 'Agrobacterium Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Brassica rapa
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    Chapter 2 Cotton (Gossypium hirsutum L.)
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    Chapter 3 Jatropha (Jatropha curcas L.).
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    Chapter 4 Sesame (Sesamum indicum L.).
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    Chapter 5 Sunflower ( Helianthus annuus L.)
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    Chapter 6 Carrot ( Daucus carota L.)
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    Chapter 7 Cassava ( Manihot esculenta Crantz)
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    Chapter 8 Potato ( Solanum tuberosum L.)
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    Chapter 9 Taro (Colocasia esculenta (L.) Schott).
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    Chapter 10 Apricot (Prunus armeniaca L.).
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    Chapter 11 Blueberry (Vaccinium corymbosum L.).
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    Chapter 12 Cherry
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    Chapter 13 Chestnut, American ( Castanea dentata (Marsh.) Borkh.)
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    Chapter 14 Chestnut, European ( Castanea sativa )
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    Chapter 15 Grapevine (Vitis vinifera L.).
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    Chapter 16 Melon ( Cucumis melo )
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    Chapter 17 Agrobacterium Protocols
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    Chapter 18 Strawberry ( Fragaria × ananassa )
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    Chapter 19 Walnut ( Juglans )
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    Chapter 20 Citrus Transformation Using Juvenile Tissue Explants
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    Chapter 21 Citrus Transformation Using Mature Tissue Explants
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    Chapter 22 Coffee (Coffea arabica L.).
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    Chapter 23 Pineapple [ Ananas comosus (L.) Merr.]
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    Chapter 24 Sugarcane (saccharum spp. Hybrids).
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    Chapter 25 Agrobacterium Protocols
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    Chapter 26 Orchids ( Oncidium and Phalaenopsis )
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    Chapter 27 Poinsettia ( Euphorbia pulcherrima Willd. ex Klotzsch)
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    Chapter 28 Populus trichocarpa
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    Chapter 29 Tall Fescue ( Festuca arundinacea Schreb.)
Attention for Chapter 15: Grapevine (Vitis vinifera L.).
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Chapter title
Grapevine (Vitis vinifera L.).
Chapter number 15
Book title
Agrobacterium Protocols
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1658-0_15
Pubmed ID
Book ISBNs
978-1-4939-1657-3, 978-1-4939-1658-0

Laurent Torregrosa, Sandrine Vialet, Angélique Adivèze, Pat Iocco-Corena, Mark R Thomas, Mark R. Thomas


Grapevine (Vitis) is considered to be one of the major fruit crops in the world based on hectares cultivated and economic value. Grapes are used not only for wine but also for fresh fruit, dried fruit, and juice production. Wine is by far the major product of grapes, and the focus of this chapter is on wine grape cultivars. Grapevine cultivars of Vitis vinifera L. have a reputation for producing premium quality wines. These premium quality wines are produced from a small number of cultivars that enjoy a high level of consumer acceptance and are firmly entrenched in the market place because of varietal name branding and the association of certain wine styles and regions with specific cultivars. In light of this situation, grapevine improvement by a transgenic approach is attractive when compared to a classical breeding approach. The transfer of individual traits as single genes with a minimum disruption to the original genome would leave the traditional characteristics of the cultivar intact. However, a reliable transformation system is required for a successful transgenic approach to grapevine improvement. There are three criteria for achieving an efficient Agrobacterium-mediated transformation system: (1) the production of highly regenerative transformable tissue, (2) optimal cocultivation conditions for both grapevine tissue and Agrobacterium, and (3) an efficient selection regime for transgenic plant regeneration. In this chapter, we describe a grapevine transformation system that meets these criteria. We also describe a protocol for the production of transformed roots suitable for functional gene studies and for the production of semi-transgenic grafted plants.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Brazil 1 2%
Unknown 42 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 23%
Researcher 8 18%
Student > Doctoral Student 5 11%
Student > Bachelor 5 11%
Student > Master 5 11%
Other 7 16%
Unknown 4 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 50%
Biochemistry, Genetics and Molecular Biology 5 11%
Social Sciences 2 5%
Veterinary Science and Veterinary Medicine 2 5%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Other 3 7%
Unknown 8 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 November 2014.
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So far Altmetric has tracked 3,224 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 408 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.