Chapter title |
Sugarcane (saccharum spp. Hybrids).
|
---|---|
Chapter number | 24 |
Book title |
Agrobacterium Protocols
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-1658-0_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1657-3, 978-1-4939-1658-0
|
Authors |
Hao Wu, Fredy Altpeter, Wu, Hao, Altpeter, Fredy |
Abstract |
Genetic transformation of sugarcane has a tremendous potential to complement traditional breeding in crop improvement and will likely transform sugarcane into a bio-factory for value-added products. We describe here Agrobacterium tumefaciens-mediated transformation of sugarcane. Embryogenic callus induced from immature leaf whorls was used as target for transformation with the hypervirulent Agrobacterium strain AGL1 carrying a constitutive nptII expression cassette in vector pPZP200. Selection with 30 mg/L geneticin during the callus phase and 30 mg/L paromomycin during regeneration of shoots and roots effectively suppressed the development of non-transgenic plants. This protocol was successful with a commercially important sugarcane cultivar, CP-88-1762, at a transformation efficiency of two independent transgenic plants per g of callus. |
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