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Agrobacterium Protocols

Overview of attention for book
Cover of 'Agrobacterium Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Brassica rapa
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    Chapter 2 Cotton (Gossypium hirsutum L.)
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    Chapter 3 Jatropha (Jatropha curcas L.).
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    Chapter 4 Sesame (Sesamum indicum L.).
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    Chapter 5 Sunflower ( Helianthus annuus L.)
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    Chapter 6 Carrot ( Daucus carota L.)
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    Chapter 7 Cassava ( Manihot esculenta Crantz)
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    Chapter 8 Potato ( Solanum tuberosum L.)
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    Chapter 9 Taro (Colocasia esculenta (L.) Schott).
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    Chapter 10 Apricot (Prunus armeniaca L.).
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    Chapter 11 Blueberry (Vaccinium corymbosum L.).
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    Chapter 12 Cherry
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    Chapter 13 Chestnut, American ( Castanea dentata (Marsh.) Borkh.)
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    Chapter 14 Chestnut, European ( Castanea sativa )
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    Chapter 15 Grapevine (Vitis vinifera L.).
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    Chapter 16 Melon ( Cucumis melo )
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    Chapter 17 Agrobacterium Protocols
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    Chapter 18 Strawberry ( Fragaria × ananassa )
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    Chapter 19 Walnut ( Juglans )
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    Chapter 20 Citrus Transformation Using Juvenile Tissue Explants
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    Chapter 21 Citrus Transformation Using Mature Tissue Explants
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    Chapter 22 Coffee (Coffea arabica L.).
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    Chapter 23 Pineapple [ Ananas comosus (L.) Merr.]
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    Chapter 24 Sugarcane (saccharum spp. Hybrids).
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    Chapter 25 Agrobacterium Protocols
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    Chapter 26 Orchids ( Oncidium and Phalaenopsis )
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    Chapter 27 Poinsettia ( Euphorbia pulcherrima Willd. ex Klotzsch)
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    Chapter 28 Populus trichocarpa
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    Chapter 29 Tall Fescue ( Festuca arundinacea Schreb.)
Attention for Chapter 24: Sugarcane (saccharum spp. Hybrids).
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Chapter title
Sugarcane (saccharum spp. Hybrids).
Chapter number 24
Book title
Agrobacterium Protocols
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1658-0_24
Pubmed ID
Book ISBNs
978-1-4939-1657-3, 978-1-4939-1658-0

Hao Wu, Fredy Altpeter


Genetic transformation of sugarcane has a tremendous potential to complement traditional breeding in crop improvement and will likely transform sugarcane into a bio-factory for value-added products. We describe here Agrobacterium tumefaciens-mediated transformation of sugarcane. Embryogenic callus induced from immature leaf whorls was used as target for transformation with the hypervirulent Agrobacterium strain AGL1 carrying a constitutive nptII expression cassette in vector pPZP200. Selection with 30 mg/L geneticin during the callus phase and 30 mg/L paromomycin during regeneration of shoots and roots effectively suppressed the development of non-transgenic plants. This protocol was successful with a commercially important sugarcane cultivar, CP-88-1762, at a transformation efficiency of two independent transgenic plants per g of callus.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 30%
Other 2 20%
Student > Bachelor 1 10%
Student > Ph. D. Student 1 10%
Lecturer > Senior Lecturer 1 10%
Other 0 0%
Unknown 2 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 40%
Linguistics 1 10%
Biochemistry, Genetics and Molecular Biology 1 10%
Environmental Science 1 10%
Engineering 1 10%
Other 0 0%
Unknown 2 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 November 2014.
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So far Altmetric has tracked 3,224 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 408 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.