Chapter title |
In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion, and Metastasis
|
---|---|
Chapter number | 13 |
Book title |
MicroRNA Protocols
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7601-0_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7600-3, 978-1-4939-7601-0
|
Authors |
Jian Xu, Xuelian Xiao, Daheng Yang, Xu, Jian, Xiao, Xuelian, Yang, Daheng |
Abstract |
MicroRNAs (miRNAs) are small noncoding, single-stranded RNAs consisting of 20-24 nucleotides (Bartel, Cell 116:281-297, 2004), which regulate target genes expression by interacting with 3'-untranslated regions (3'-UTRs) of target mRNAs, leading to translation repression or mRNA degradation (Filipowicz et al., Nat Rev Genet 9:102-114, 2008; Nilsen, Trends Genet 23:243-249, 2007). Accumulating evidence has elucidated them as oncogenes or tumor suppressors in cancers such as hepatocellular carcinoma, breast cancer, and lung cancer (Liu et al., Gastroenterology 136:683-693, 2009; Yu et al., Cell 131:1109-1123, 2007; Zhou et al., Sci Rep 7:42680, 2017; Iorio and Croce, Carcinogenesis 33:1126-1133, 2012). MiRNAs are involved in various biological processes, including cell proliferation (Liu et al., Mol Cancer Res 11:1314-1325, 2013), differentiation (Liu et al., Mol Cancer Res 11:1314-1325, 2013), apoptosis (Pan et al., Oncol Res 24:429-435, 2016), invasion and metastasis (Liu et al., Nat Commun 8:14270, 2017). Understanding the role of miRNAs in tumor gives new perspective on cancer diagnosis and therapy (Rupaimoole and Slack, Nat Rev Drug Discov 16:203-222, 2017; Berindan-Neagoe et al., CA Cancer J Clin 64:311-336, 2014). This chapter will focus on the in vitro methods for identifying miRNAs roles in cell proliferation, invasion, and metastasis in tumor development, which includes CCK-8 assay, Wound Healing assay, and Transwell assay. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 29% |
Researcher | 4 | 29% |
Librarian | 1 | 7% |
Student > Doctoral Student | 1 | 7% |
Unknown | 4 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 3 | 21% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Agricultural and Biological Sciences | 2 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Medicine and Dentistry | 1 | 7% |
Other | 1 | 7% |
Unknown | 4 | 29% |