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MicroRNA Protocols

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Cover of 'MicroRNA Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 The MicroRNA
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    Chapter 2 Target mRNA-Driven Biogenesis of Cognate MicroRNAs In Vitro
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    Chapter 3 Isolation of Viral-Infected Brain Regions for miRNA Profiling from Formalin-Fixed Paraffin-Embedded Tissues by Laser Capture Microdissection
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    Chapter 4 Isolation and Analysis of Exosomal MicroRNAs from Ovarian Follicular Fluid
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    Chapter 5 Profiling of MicroRNAs in the Biofluids of Livestock Species
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    Chapter 6 Exosomal MicroRNAs as Potential Biomarkers in Neuropsychiatric Disorders
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    Chapter 7 Identification and Validation of Potential Differential miRNA Regulation via Alternative Polyadenylation
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    Chapter 8 How to Explore the Function and Importance of MicroRNAs: MicroRNAs Expression Profile and Their Target/Pathway Prediction in Bovine Ovarian Cells
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    Chapter 9 Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs
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    Chapter 10 Mining Exosomal MicroRNAs from Human-Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration
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    Chapter 11 Quantitative Analysis of Precursors MicroRNAs and Their Respective Mature MicroRNAs in Cancer Exosomes Overtime
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    Chapter 12 Quantum Language of MicroRNA: Application for New Cancer Therapeutic Targets
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    Chapter 13 In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion, and Metastasis
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    Chapter 14 Isolation and Identification of Gene-Specific MicroRNAs
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    Chapter 15 Comprehensive Measurement of Gene Silencing Involving Endogenous MicroRNAs in Mammalian Cells
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    Chapter 16 Screening miRNA for Functional Significance by 3D Cell Culture System
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    Chapter 17 Neonatal Rat Cardiomyocytes Isolation, Culture, and Determination of MicroRNAs’ Effects in Proliferation
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    Chapter 18 Gene Manipulation with Micro RNAs at Single-Human Cancer Cell
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    Chapter 19 Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis
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    Chapter 20 Transgene-Like Animal Models Using Intronic MicroRNAs
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    Chapter 21 Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming
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    Chapter 22 Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR-302 Induction
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    Chapter 23 The miR-302-Mediated Induction of Pluripotent Stem Cells (iPSC): Multiple Synergistic Reprogramming Mechanisms
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    Chapter 24 Identification and Isolation of Novel Sugar-Like RNA Protecting Materials: Glycylglycerins from Pluripotent Stem Cells
Attention for Chapter 13: In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion, and Metastasis
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Chapter title
In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion, and Metastasis
Chapter number 13
Book title
MicroRNA Protocols
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7601-0_13
Pubmed ID
Book ISBNs
978-1-4939-7600-3, 978-1-4939-7601-0
Authors

Jian Xu, Xuelian Xiao, Daheng Yang, Xu, Jian, Xiao, Xuelian, Yang, Daheng

Abstract

MicroRNAs (miRNAs) are small noncoding, single-stranded RNAs consisting of 20-24 nucleotides (Bartel, Cell 116:281-297, 2004), which regulate target genes expression by interacting with 3'-untranslated regions (3'-UTRs) of target mRNAs, leading to translation repression or mRNA degradation (Filipowicz et al., Nat Rev Genet 9:102-114, 2008; Nilsen, Trends Genet 23:243-249, 2007). Accumulating evidence has elucidated them as oncogenes or tumor suppressors in cancers such as hepatocellular carcinoma, breast cancer, and lung cancer (Liu et al., Gastroenterology 136:683-693, 2009; Yu et al., Cell 131:1109-1123, 2007; Zhou et al., Sci Rep 7:42680, 2017; Iorio and Croce, Carcinogenesis 33:1126-1133, 2012). MiRNAs are involved in various biological processes, including cell proliferation (Liu et al., Mol Cancer Res 11:1314-1325, 2013), differentiation (Liu et al., Mol Cancer Res 11:1314-1325, 2013), apoptosis (Pan et al., Oncol Res 24:429-435, 2016), invasion and metastasis (Liu et al., Nat Commun 8:14270, 2017). Understanding the role of miRNAs in tumor gives new perspective on cancer diagnosis and therapy (Rupaimoole and Slack, Nat Rev Drug Discov 16:203-222, 2017; Berindan-Neagoe et al., CA Cancer J Clin 64:311-336, 2014). This chapter will focus on the in vitro methods for identifying miRNAs roles in cell proliferation, invasion, and metastasis in tumor development, which includes CCK-8 assay, Wound Healing assay, and Transwell assay.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 29%
Researcher 4 29%
Librarian 1 7%
Student > Doctoral Student 1 7%
Unknown 4 29%
Readers by discipline Count As %
Neuroscience 3 21%
Biochemistry, Genetics and Molecular Biology 2 14%
Agricultural and Biological Sciences 2 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Medicine and Dentistry 1 7%
Other 1 7%
Unknown 4 29%