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MicroRNA Protocols

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Cover of 'MicroRNA Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 The MicroRNA
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    Chapter 2 Target mRNA-Driven Biogenesis of Cognate MicroRNAs In Vitro
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    Chapter 3 Isolation of Viral-Infected Brain Regions for miRNA Profiling from Formalin-Fixed Paraffin-Embedded Tissues by Laser Capture Microdissection
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    Chapter 4 Isolation and Analysis of Exosomal MicroRNAs from Ovarian Follicular Fluid
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    Chapter 5 Profiling of MicroRNAs in the Biofluids of Livestock Species
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    Chapter 6 Exosomal MicroRNAs as Potential Biomarkers in Neuropsychiatric Disorders
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    Chapter 7 Identification and Validation of Potential Differential miRNA Regulation via Alternative Polyadenylation
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    Chapter 8 How to Explore the Function and Importance of MicroRNAs: MicroRNAs Expression Profile and Their Target/Pathway Prediction in Bovine Ovarian Cells
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    Chapter 9 Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs
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    Chapter 10 Mining Exosomal MicroRNAs from Human-Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration
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    Chapter 11 Quantitative Analysis of Precursors MicroRNAs and Their Respective Mature MicroRNAs in Cancer Exosomes Overtime
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    Chapter 12 Quantum Language of MicroRNA: Application for New Cancer Therapeutic Targets
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    Chapter 13 In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion, and Metastasis
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    Chapter 14 Isolation and Identification of Gene-Specific MicroRNAs
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    Chapter 15 Comprehensive Measurement of Gene Silencing Involving Endogenous MicroRNAs in Mammalian Cells
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    Chapter 16 Screening miRNA for Functional Significance by 3D Cell Culture System
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    Chapter 17 Neonatal Rat Cardiomyocytes Isolation, Culture, and Determination of MicroRNAs’ Effects in Proliferation
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    Chapter 18 Gene Manipulation with Micro RNAs at Single-Human Cancer Cell
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    Chapter 19 Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis
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    Chapter 20 Transgene-Like Animal Models Using Intronic MicroRNAs
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    Chapter 21 Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming
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    Chapter 22 Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR-302 Induction
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    Chapter 23 The miR-302-Mediated Induction of Pluripotent Stem Cells (iPSC): Multiple Synergistic Reprogramming Mechanisms
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    Chapter 24 Identification and Isolation of Novel Sugar-Like RNA Protecting Materials: Glycylglycerins from Pluripotent Stem Cells
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Chapter title
How to Explore the Function and Importance of MicroRNAs: MicroRNAs Expression Profile and Their Target/Pathway Prediction in Bovine Ovarian Cells
Chapter number 8
Book title
MicroRNA Protocols
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7601-0_8
Pubmed ID
Book ISBNs
978-1-4939-7600-3, 978-1-4939-7601-0
Authors

Anna E. Zielak-Steciwko, John A. Browne, Zielak-Steciwko, Anna E., Browne, John A.

Abstract

Micro RNAs (miRNA) are integral components of genetic regulatory networks and act by binding to the transcripts of their corresponding target genes, leading to a decrease in protein production levels either by mRNA degradation or by translational repression. While the role of miRNAs is ubiquitous, they have a particular importance with regard to cell differentiation. The miRNA-target mRNA interaction has a significant impact on many signaling pathways and the cross-talk between them; playing a regulatory role in a variety of different physiological processes within the cells. Ovarian follicle development is a physiological process that is not fully understood with regard to miRNA regulation; there are many questions that remain with respect to the molecular regulation of this important process. Bovine follicular cells are a good experimental model for the investigation of these mechanisms, having direct implications on reproductive health in humans. This chapter describes how differentially expressed miRNAs are identified in the granulosa and theca cells of dominant and subordinate bovine ovarian follicles and the identification of their associated targets and pathways. This chapter systematically describes how the granulosa and theca cells are dissected from the ovarian follicles. Afterward, we present a detailed protocol for miRNA extraction, based on a combined TRI reagent/column clean-up method, and also miRNA expression profiling using both microarray and RT-qPCR. In addition, an outline is provided of the bioinformatic analysis which enables the prediction of miRNAs targets. Pathways associated with the differentially expressed miRNAs are also elucidated using DIANA-miRPath software.

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Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 40%
Student > Postgraduate 1 20%
Student > Doctoral Student 1 20%
Unknown 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 20%
Agricultural and Biological Sciences 1 20%
Chemistry 1 20%
Medicine and Dentistry 1 20%
Unknown 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 January 2019.
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#18,603,172
of 23,043,346 outputs
Outputs from Methods in molecular biology
#7,992
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#330,621
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Outputs of similar age from Methods in molecular biology
#950
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