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Caspases,Paracaspases, and Metacaspases

Overview of attention for book
Attention for Chapter 7: Detecting caspase activity in Drosophila larval imaginal discs.
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Chapter title
Detecting caspase activity in Drosophila larval imaginal discs.
Chapter number 7
Book title
Caspases,Paracaspases, and Metacaspases
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-4939-0357-3_7
Pubmed ID
Book ISBNs
978-1-4939-0356-6, 978-1-4939-0357-3
Authors

Fogarty CE, Bergmann A, Caitlin E. Fogarty, Andreas Bergmann

Abstract

Caspases are a highly specialized class of cell death proteases. Since they are synthesized as inactive full-length zymogens, activation--at least of effector caspases and to some extent also of initiator caspases-requires a proteolytic cleavage event, generating a large and a small subunit, two of each forming the active caspase. The proteolytic cleavage event generates neo-epitopes at both the C-terminus of the large subunit and the N-terminus of the small subunit. The cleaved Caspase-3 (CC3) antibody was raised against the neo-epitope of the large subunit and thus detects only cleaved, but not full-length, Caspase-3. Although raised against human cleaved Caspase-3, the CC3 antibody cross-reacts in other species and detects cleaved caspases, most notably DrICE and Dcp-1, in Drosophila. This protocol describes the procedure for use of the CC3 antibody to detect caspase activity in larval imaginal discs in Drosophila.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 20%
Professor > Associate Professor 1 20%
Student > Bachelor 1 20%
Student > Master 1 20%
Unknown 1 20%
Readers by discipline Count As %
Medicine and Dentistry 2 40%
Immunology and Microbiology 1 20%
Biochemistry, Genetics and Molecular Biology 1 20%
Unknown 1 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 February 2014.
All research outputs
#2,898,553
of 3,631,524 outputs
Outputs from Methods in molecular biology
#1,434
of 2,358 outputs
Outputs of similar age
#73,837
of 92,889 outputs
Outputs of similar age from Methods in molecular biology
#88
of 124 outputs
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