↓ Skip to main content

Caspases,Paracaspases, and Metacaspases

Overview of attention for book
Attention for Chapter 7: Detecting caspase activity in Drosophila larval imaginal discs.
Altmetric Badge

Citations

dimensions_citation
7 Dimensions

Readers on

mendeley
8 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Detecting caspase activity in Drosophila larval imaginal discs.
Chapter number 7
Book title
Caspases,Paracaspases, and Metacaspases
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-4939-0357-3_7
Pubmed ID
Book ISBNs
978-1-4939-0356-6, 978-1-4939-0357-3
Authors

Fogarty CE, Bergmann A, Caitlin E. Fogarty, Andreas Bergmann, Fogarty, Caitlin E., Bergmann, Andreas

Abstract

Caspases are a highly specialized class of cell death proteases. Since they are synthesized as inactive full-length zymogens, activation--at least of effector caspases and to some extent also of initiator caspases-requires a proteolytic cleavage event, generating a large and a small subunit, two of each forming the active caspase. The proteolytic cleavage event generates neo-epitopes at both the C-terminus of the large subunit and the N-terminus of the small subunit. The cleaved Caspase-3 (CC3) antibody was raised against the neo-epitope of the large subunit and thus detects only cleaved, but not full-length, Caspase-3. Although raised against human cleaved Caspase-3, the CC3 antibody cross-reacts in other species and detects cleaved caspases, most notably DrICE and Dcp-1, in Drosophila. This protocol describes the procedure for use of the CC3 antibody to detect caspase activity in larval imaginal discs in Drosophila.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 25%
Professor > Associate Professor 2 25%
Professor 1 13%
Student > Bachelor 1 13%
Student > Postgraduate 1 13%
Other 0 0%
Unknown 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Medicine and Dentistry 2 25%
Neuroscience 1 13%
Immunology and Microbiology 1 13%
Unknown 1 13%