| Chapter title |
Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC
|
|---|---|
| Chapter number | 16 |
| Book title |
Caspases,Paracaspases, and Metacaspases
|
| Published in |
Methods in molecular biology, February 2014
|
| DOI | 10.1007/978-1-4939-0357-3_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-0356-6, 978-1-4939-0357-3
|
| Authors |
Tsiatsiani, Liana, Stael, Simon, Van Damme, Petra, Van Breusegem, Frank, Gevaert, Kris, Liana Tsiatsiani, Simon Stael, Petra Van Damme, Frank Van Breusegem, Kris Gevaert, Damme, Petra, Breusegem, Frank, Damme, Petra Van, Breusegem, Frank Van |
| Abstract |
Proteome-wide discovery of in vivo metacaspase substrates can be obtained by positional proteomics approaches such as N-terminal COFRADIC, for example by comparing the N-terminal proteomes (or N-terminomes) of wild-type plants to transgenic plants not expressing a given metacaspase. In this chapter we describe a protocol for the preparation of plant tissue proteomes, including differential isotopic labelling allowing for a comparison of in vivo N-terminomes that serves as the starting point for N-terminal COFRADIC studies. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 40% |
| Researcher | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Student > Master | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Nursing and Health Professions | 1 | 20% |
| Agricultural and Biological Sciences | 1 | 20% |
| Unknown | 2 | 40% |