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Mendeley readers
| Chapter title |
Non-radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length
|
|---|---|
| Chapter number | 17 |
| Book title |
Chromatin Protocols
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2474-5_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2473-8, 978-1-4939-2474-5
|
| Authors |
Partha P. Banerjee, Shankar Jagadeesh |
| Abstract |
The Telomeric repeat amplification protocol (TRAP) is a highly sensitive PCR-based assay and prove to be an important tool for understanding the role of telomerase in cancer and various tissues that harbors telomerase positive stem cells. This assay measures telomerase activity where the amount of target is dependent upon the activity of the enzyme. This protocol consists of two steps: first, telomeric repeats are added to the substrate by telomerase present in the cell and second, the extended products are amplified by Taq-DNA polymerase. The amplified TRAP products are separated on 10 % native PAGE and detected by SYBR Green I dye. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 17% |
| Student > Master | 2 | 17% |
| Researcher | 2 | 17% |
| Professor > Associate Professor | 1 | 8% |
| Unknown | 5 | 42% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Agricultural and Biological Sciences | 2 | 17% |
| Medicine and Dentistry | 1 | 8% |
| Unknown | 6 | 50% |