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Chromatin Protocols

Overview of attention for book
Cover of 'Chromatin Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support.
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    Chapter 2 Preparation and analysis of positioned mononucleosomes.
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    Chapter 3 Chromatin imaging with time-lapse atomic force microscopy.
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    Chapter 4 Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using CRISPR.
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    Chapter 5 Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin.
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    Chapter 6 Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes.
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    Chapter 7 Histone Deacetylase Activity Assay
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    Chapter 8 In vitro histone demethylase assays.
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    Chapter 9 Integrated DNA methylation and chromatin structural analysis at single-molecule resolution.
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    Chapter 10 Determination of DNA Methylation Levels Using Illumina HumanMethylation450 BeadChips.
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    Chapter 11 Investigation of genomic methylation status using methylation-specific and bisulfite sequencing polymerase chain reaction.
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    Chapter 12 In vitro and in vivo assays for studying histone ubiquitination and deubiquitination.
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    Chapter 13 Immunostaining analysis of tissue cultured cells and tissue sections using phospho-histone h3 (serine 10) antibody.
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    Chapter 14 Identification and characterization of nonhistone chromatin proteins: human positive coactivator 4 as a candidate.
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    Chapter 15 Methods to study transcription-coupled repair in chromatin.
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    Chapter 16 Analysis of DNA replication associated chromatin decondensation: in vivo assay for understanding chromatin remodeling mechanisms of selected proteins.
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    Chapter 17 Non-radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length
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    Chapter 18 Detecting ATM-Dependent Chromatin Modification in DNA Damage Response.
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    Chapter 19 Imaging Local Deposition of Newly Synthesized Histones in UVC-Damaged Chromatin.
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    Chapter 20 In vitro replication assay with Mammalian cell extracts.
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    Chapter 21 Fluorescent In Situ Hybridization on Comets: FISH Comet.
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    Chapter 22 Methods to study histone chaperone function in nucleosome assembly and chromatin transcription.
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    Chapter 23 Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase Using spFRET.
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    Chapter 24 Transcriptome-Wide Identification of In Vivo Interactions Between RNAs and RNA-Binding Proteins by RIP and PAR-CLIP Assays.
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    Chapter 25 Chromatin immunoprecipitation assays: analyzing transcription factor binding and histone modifications in vivo.
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    Chapter 26 ChIP on Chip and ChIP-Seq Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications.
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    Chapter 27 ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays.
Attention for Chapter 6: Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes.
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Chapter title
Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes.
Chapter number 6
Book title
Chromatin Protocols
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2474-5_6
Pubmed ID
25827876
Book ISBNs
978-1-4939-2473-8, 978-1-4939-2474-5
Authors

Carless, Melanie A, Melanie A. Carless

Abstract

Comparative genomic hybridization (CGH) allows the global screening of copy number aberrations within a sample. Specifically, large (>20 mb) deletions and amplifications are detected, based on utilization of test and reference (karyotypically normal) DNA. These samples are whole-genome amplified by DOP-PCR and then differentially labeled with fluorophores via nick translation. Test and reference samples are competitively hybridized to normal metaphase chromosomes. The relative amount of each DNA that binds to a chromosomal locus is indicative of the abundance of that DNA. Thus, if a chromosomal region is amplified, the test DNA will out-compete the reference DNA for binding and fluorescence will indicate amplification. Conversely, if a region is deleted, more reference DNA will bind and fluorescence will indicate a deletion. The following chapter outlines the protocols used for CGH analysis of metaphase chromosomes. These protocols include metaphase chromosome slide preparation, DNA extraction (from blood, cell lines, and microdissected formalin-fixed paraffin-embedded tissue), DOP-PCR, nick translation, in situ hybridization, and fluorescence microscopy and image analysis.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 60%
Other 1 20%
Professor 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 60%
Agricultural and Biological Sciences 1 20%
Medicine and Dentistry 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2015.
All research outputs
#18,432,465
of 22,835,198 outputs
Outputs from Methods in molecular biology
#7,920
of 13,126 outputs
Outputs of similar age
#255,939
of 353,204 outputs
Outputs of similar age from Methods in molecular biology
#481
of 997 outputs
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So far Altmetric has tracked 13,126 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

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