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Chromatin Protocols

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Cover of 'Chromatin Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support.
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    Chapter 2 Preparation and analysis of positioned mononucleosomes.
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    Chapter 3 Chromatin imaging with time-lapse atomic force microscopy.
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    Chapter 4 Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using CRISPR.
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    Chapter 5 Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin.
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    Chapter 6 Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes.
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    Chapter 7 Histone Deacetylase Activity Assay
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    Chapter 8 In vitro histone demethylase assays.
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    Chapter 9 Integrated DNA methylation and chromatin structural analysis at single-molecule resolution.
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    Chapter 10 Determination of DNA Methylation Levels Using Illumina HumanMethylation450 BeadChips.
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    Chapter 11 Investigation of genomic methylation status using methylation-specific and bisulfite sequencing polymerase chain reaction.
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    Chapter 12 In vitro and in vivo assays for studying histone ubiquitination and deubiquitination.
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    Chapter 13 Immunostaining analysis of tissue cultured cells and tissue sections using phospho-histone h3 (serine 10) antibody.
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    Chapter 14 Identification and characterization of nonhistone chromatin proteins: human positive coactivator 4 as a candidate.
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    Chapter 15 Methods to study transcription-coupled repair in chromatin.
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    Chapter 16 Analysis of DNA replication associated chromatin decondensation: in vivo assay for understanding chromatin remodeling mechanisms of selected proteins.
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    Chapter 17 Non-radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length
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    Chapter 18 Detecting ATM-Dependent Chromatin Modification in DNA Damage Response.
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    Chapter 19 Imaging Local Deposition of Newly Synthesized Histones in UVC-Damaged Chromatin.
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    Chapter 20 In vitro replication assay with Mammalian cell extracts.
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    Chapter 21 Fluorescent In Situ Hybridization on Comets: FISH Comet.
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    Chapter 22 Methods to study histone chaperone function in nucleosome assembly and chromatin transcription.
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    Chapter 23 Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase Using spFRET.
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    Chapter 24 Transcriptome-Wide Identification of In Vivo Interactions Between RNAs and RNA-Binding Proteins by RIP and PAR-CLIP Assays.
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    Chapter 25 Chromatin immunoprecipitation assays: analyzing transcription factor binding and histone modifications in vivo.
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    Chapter 26 ChIP on Chip and ChIP-Seq Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications.
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    Chapter 27 ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays.
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