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Detection of Blotted Proteins

Overview of attention for book
Cover of 'Detection of Blotted Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Western Blotting: Origin and Ascent of the Species.
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    Chapter 2 Methods to Concentrate Proteins for Protein Isolation, Proteomic, and Peptidomic Evaluation.
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    Chapter 3 Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose
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    Chapter 4 Detection of Blotted Proteins: Not All Blockers Are Created Equal
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    Chapter 5 Protein Stains to Detect Antigen on Membranes
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    Chapter 6 Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting
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    Chapter 7 Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation
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    Chapter 8 Use of Nonradioactive Detection Method for North- and South-Western Blot
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    Chapter 9 Immunoblotting Using Radiolabeled Reagents for Detection
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    Chapter 10 Immunoblotting of Antigens: Whole, Strip, and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique
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    Chapter 11 Detection of Protein Carbonyls by Means of Biotin Hydrazide–Streptavidin Affinity Methods
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    Chapter 12 Direct Immunodetection of Antigens Within the Precast Polyacrylamide Gel
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    Chapter 13 Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays
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    Chapter 14 Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
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    Chapter 15 Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL Plex Immunoblotting in a Standard Proteomics Workflow.
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    Chapter 16 Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot Blots, and Immunohistochemistry
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    Chapter 17 Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane
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    Chapter 18 Sequential Use of Immunoblots for Characterization of Autoantibody Specificities
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    Chapter 19 Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western Blotting
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    Chapter 20 Application of Intermittent Microwave Irradiation to Western Blot Analysis
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    Chapter 21 Visualization of Unstained Protein Bands on PVDF
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    Chapter 22 Multiplexed Fluorescent Immunodetection Using Low Autofluorescence Immobilon ® -FL Membrane
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    Chapter 23 Cold Microwave-Enabled Protein Detection and Quantification
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    Chapter 24 TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics
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    Chapter 25 Analysis of Electroblotted Proteins by Mass Spectrometry.
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    Chapter 26 On-Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions
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    Chapter 27 Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore
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    Chapter 28 Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane
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    Chapter 29 Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta
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    Chapter 30 Nonstripping “Rainbow” and Multiple Antigen Detection (MAD) Western Blotting
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    Chapter 31 Supported Molecular Matrix Electrophoresis
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    Chapter 32 Parafilm-M(®), An Available Cost-Effective Alternative for Immuno-blot Pouches.
  34. Altmetric Badge
    Chapter 33 Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes
  35. Altmetric Badge
    Chapter 34 Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva
  36. Altmetric Badge
    Chapter 35 A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMB
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    Chapter 36 Other Notable Methods of Membrane Protein Detection: A Brief Review
  38. Altmetric Badge
    Chapter 37 Nitrocellulose Membrane: The New Canvas
  39. Altmetric Badge
    Chapter 38 Invisible Ink Marking in ECL Membrane Assays.
Attention for Chapter 2: Methods to Concentrate Proteins for Protein Isolation, Proteomic, and Peptidomic Evaluation.
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Chapter title
Methods to Concentrate Proteins for Protein Isolation, Proteomic, and Peptidomic Evaluation.
Chapter number 2
Book title
Detection of Blotted Proteins
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2718-0_2
Pubmed ID
Book ISBNs
978-1-4939-2717-3, 978-1-4939-2718-0
Authors

Goldring, J P Dean, J. P. Dean Goldring

Abstract

In protein isolation, proteomic, or peptidomic procedures, protein solutions are often concentrated to remove solvents and undesirable molecules, to separate protein fractions or to increase protein concentrations. Proteins can be concentrated by precipitation from solution with ammonium sulfate, polyethylene glycol, organic solvent, trichloroacetic acid, potassium chloride/sodium dodecyl sulfate, and three-phase partitioning. Solvents can be removed by passage through a semipermeable barrier where protein solutions are forced against the barrier in a centrifuge tube or with increased pressure concentrating protein in the remaining solution. The semipermeable barrier can be surrounded by a hygroscopic reagent to draw the solvent across the membrane. Proteins can be concentrated by freeze-drying (lyophilization). All these methods to concentrate proteins are discussed.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 10 38%
Researcher 3 12%
Student > Ph. D. Student 3 12%
Other 2 8%
Student > Master 2 8%
Other 3 12%
Unknown 3 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 42%
Agricultural and Biological Sciences 3 12%
Immunology and Microbiology 2 8%
Chemical Engineering 1 4%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 1 4%
Unknown 7 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 July 2015.
All research outputs
#3,520,362
of 5,313,386 outputs
Outputs from Methods in molecular biology
#1,820
of 4,163 outputs
Outputs of similar age
#121,932
of 187,669 outputs
Outputs of similar age from Methods in molecular biology
#65
of 152 outputs
Altmetric has tracked 5,313,386 research outputs across all sources so far. This one is in the 29th percentile – i.e., 29% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,163 research outputs from this source. They receive a mean Attention Score of 1.5. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 187,669 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 152 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.