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Detection of Blotted Proteins

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Cover of 'Detection of Blotted Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Western Blotting: Origin and Ascent of the Species.
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    Chapter 2 Methods to Concentrate Proteins for Protein Isolation, Proteomic, and Peptidomic Evaluation.
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    Chapter 3 Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose
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    Chapter 4 Detection of Blotted Proteins: Not All Blockers Are Created Equal
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    Chapter 5 Protein Stains to Detect Antigen on Membranes
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    Chapter 6 Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting
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    Chapter 7 Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation
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    Chapter 8 Use of Nonradioactive Detection Method for North- and South-Western Blot
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    Chapter 9 Immunoblotting Using Radiolabeled Reagents for Detection
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    Chapter 10 Immunoblotting of Antigens: Whole, Strip, and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique
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    Chapter 11 Detection of Protein Carbonyls by Means of Biotin Hydrazide–Streptavidin Affinity Methods
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    Chapter 12 Direct Immunodetection of Antigens Within the Precast Polyacrylamide Gel
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    Chapter 13 Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays
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    Chapter 14 Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
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    Chapter 15 Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL Plex Immunoblotting in a Standard Proteomics Workflow.
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    Chapter 16 Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot Blots, and Immunohistochemistry
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    Chapter 17 Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane
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    Chapter 18 Sequential Use of Immunoblots for Characterization of Autoantibody Specificities
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    Chapter 19 Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western Blotting
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    Chapter 20 Application of Intermittent Microwave Irradiation to Western Blot Analysis
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    Chapter 21 Visualization of Unstained Protein Bands on PVDF
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    Chapter 22 Multiplexed Fluorescent Immunodetection Using Low Autofluorescence Immobilon ® -FL Membrane
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    Chapter 23 Cold Microwave-Enabled Protein Detection and Quantification
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    Chapter 24 TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics
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    Chapter 25 Analysis of Electroblotted Proteins by Mass Spectrometry.
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    Chapter 26 On-Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions
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    Chapter 27 Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore
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    Chapter 28 Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane
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    Chapter 29 Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta
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    Chapter 30 Nonstripping “Rainbow” and Multiple Antigen Detection (MAD) Western Blotting
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    Chapter 31 Supported Molecular Matrix Electrophoresis
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    Chapter 32 Parafilm-M(®), An Available Cost-Effective Alternative for Immuno-blot Pouches.
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    Chapter 33 Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes
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    Chapter 34 Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva
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    Chapter 35 A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMB
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    Chapter 36 Other Notable Methods of Membrane Protein Detection: A Brief Review
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    Chapter 37 Nitrocellulose Membrane: The New Canvas
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    Chapter 38 Invisible Ink Marking in ECL Membrane Assays.
Attention for Chapter 38: Invisible Ink Marking in ECL Membrane Assays.
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Chapter title
Invisible Ink Marking in ECL Membrane Assays.
Chapter number 38
Book title
Detection of Blotted Proteins
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2718-0_38
Pubmed ID
Book ISBNs
978-1-4939-2717-3, 978-1-4939-2718-0
Authors

Kurien, Biji T, Biji T. Kurien, Kurien, Biji T.

Abstract

Invisible ink and writing secret messages have been part of man's fantasy, having proven useful in clandestine and high sensitivity areas. Security inks, made up of invisible materials that give printed, anti-photocopy images capable of being read only under special environments, have become important. An ink formulation based on silicon(IV) 2,3-naphthalocyanine bis(trihexylsilyloxide) as colorant, invisible to the naked eye but infrared readable, has been described earlier. Biometric DNA ink has also been developed for security authentication. In lighter vein, many budding scientists and others have often experimented with writing secret messages on paper, either for purposes of fun or actually sending secret messages to friends. It involved the use of lemon juice, milk, or other solutions that could be used with a dip pen, brush, or a fountain pen to write invisible messages on a blank white paper. Words turn up as magic when the paper is exposed to heat in one form or the other. Here, we attempt to end this book on a slightly humorous note by showing that invisible messages can be written on nitrocellulose membranes (but not on polyvinylidene difluoride membranes) using an appropriately diluted horse radish peroxidase/alkaline phosphatase anti-IgG conjugate (rabbit, mouse, or human anti-IgG). The message is written on the membrane, preferably with a fountain pen, and the membrane is allowed to dry. Regular detection with enhanced chemiluminescence plus or nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate systems is used to unravel the secret message. In addition, this method could be used to mark nitrocellulose membranes for orientation purposes using ECL detection system and thus can eliminate the use of autoradiography pens.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Researcher 1 33%
Student > Master 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 67%
Immunology and Microbiology 1 33%