| Chapter title |
In-Cell NMR in Xenopus laevis Oocytes.
|
|---|---|
| Chapter number | 3 |
| Book title |
Intrinsically Disordered Protein Analysis
|
| Published in |
Methods in molecular biology, July 2012
|
| DOI | 10.1007/978-1-61779-927-3_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-926-6, 978-1-61779-927-3
|
| Authors |
Thongwichian R, Selenko P, Rossukon Thongwichian, Philipp Selenko |
| Abstract |
For the purpose of studying IDPs inside cells of higher organisms, several eukaryotic in-cell NMR systems have been developed over the past years. In this chapter we will focus on high-resolution in-cell NMR applications in Xenopus laevis oocytes, the first eukaryotic cellular model system to be established. In contrast to prokaryotic in-cell NMR samples, eukaryotic in-cell NMR specimens are prepared by cytoplasmic delivery of an exogenously produced, isotope-labeled protein into the non-isotope-labeled environment of the respective "host" cell. In-cell NMR applications in Xenopus oocytes rely on intracellular sample deposition by direct microinjection into the oocyte cytoplasm. Here, we describe the preparation of oocyte in-cell NMR samples for IDP studies in this cellular model environment. |
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