Chapter title |
Quantitation of 25-OH-Vitamin-D 2 and 25-OH-Vitamin-D 3 in Urine Using LC-MS/MS
|
---|---|
Chapter number | 33 |
Book title |
Clinical Applications of Mass Spectrometry in Biomolecular Analysis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3182-8_33 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3181-1, 978-1-4939-3182-8
|
Authors |
Dean C. Carlow, Ryan C. Schofield, Michelle Denburg |
Abstract |
Patients with significant proteinuria represent a unique population with respect to vitamin D status due to the urinary losses of vitamin D-binding protein (DBP) to which >99 % of circulating 25-hydroxy vitamin D (25(OH)D) is bound. Low serum concentrations of 25(OH)D have been found in children and adults with nephrotic syndrome (NS). However, previously described assays developed to quantify the magnitude of urinary loss are technically challenging. This chapter describes a simple and sensitive method to quantify 25(OH)D2 and 25(OH)D3 in urine specimens in a single analytical LC-MS/MS analysis. This assay is more sensitive than previously described radioimmunoassays and offers the ability to quantitate both forms of 25-hydroxy vitamin D. The assay involves no chemical derivitization, has a linear measurement range of 20-1500 pg/mL and displays imprecision (CVs) below 7 % at various concentrations across the analytical measurement range. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 35 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 20% |
Student > Bachelor | 4 | 11% |
Professor > Associate Professor | 4 | 11% |
Student > Doctoral Student | 3 | 9% |
Other | 3 | 9% |
Other | 8 | 23% |
Unknown | 6 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 7 | 20% |
Computer Science | 6 | 17% |
Medicine and Dentistry | 4 | 11% |
Biochemistry, Genetics and Molecular Biology | 2 | 6% |
Chemistry | 2 | 6% |
Other | 6 | 17% |
Unknown | 8 | 23% |