Chapter title |
Quantification of Free Carnitine and Acylcarnitines in Plasma or Serum Using HPLC/MS/MS
|
---|---|
Chapter number | 2 |
Book title |
Clinical Applications of Mass Spectrometry in Biomolecular Analysis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3182-8_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3181-1, 978-1-4939-3182-8
|
Authors |
David Scott, Bryce Heese, Uttam Garg |
Abstract |
Acylcarnitines are formed by esterification between fatty acids CoA or organic acids CoA molecules and carnitine. In various fatty acids oxidation defects and organic acidurias, there is increased concentration of corresponding acylcarnitines. Abnormalities in specific acylcarnitines are used in the diagnosis of fatty acids oxidation defects and organic acidurias. Most commonly used method for the assay of acylcarnitines is HPLC-tandem mass spectrometry (HPLC/MS/MS). A HPLC/MS/MS method is described for the quantification of number of acylcarnitines. The method involves butylation of carnitine/acylcarnitines using acidified butanol, HPLC flow injection, and measurement of acylcarnitines using precursor ion scan and multiple reactions monitoring (MRM). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 36% |
Student > Doctoral Student | 2 | 14% |
Student > Bachelor | 2 | 14% |
Lecturer > Senior Lecturer | 1 | 7% |
Student > Ph. D. Student | 1 | 7% |
Other | 1 | 7% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 21% |
Biochemistry, Genetics and Molecular Biology | 3 | 21% |
Agricultural and Biological Sciences | 3 | 21% |
Medicine and Dentistry | 2 | 14% |
Business, Management and Accounting | 1 | 7% |
Other | 1 | 7% |
Unknown | 1 | 7% |