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Clinical Applications of Mass Spectrometry in Biomolecular Analysis

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Cover of 'Clinical Applications of Mass Spectrometry in Biomolecular Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Mass Spectrometry in Clinical Laboratory: Applications in Biomolecular Analysis
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    Chapter 2 Quantification of Free Carnitine and Acylcarnitines in Plasma or Serum Using HPLC/MS/MS
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    Chapter 3 Quantification of Arginine and Its Methylated Derivatives in Plasma by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 4 Quantitation of Albumin in Urine by Liquid Chromatography Tandem Mass Spectrometry
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    Chapter 5 Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 6 Quantification of Five Clinically Important Amino Acids by HPLC-Triple TOF™ 5600 Based on Pre-column Double Derivatization Method
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    Chapter 7 Sensitive, Simple, and Robust Nano-Liquid Chromatography-Mass Spectrometry Method for Amyloid Protein Subtyping
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    Chapter 8 Quantitation of Ubiquinone (Coenzyme Q 10 ) in Serum/Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (ESI-LC-MS/MS)
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    Chapter 9 Quantitative Analysis of Salivary Cortisol Using LC-MS/MS
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    Chapter 10 Quantification of Dihydroxyacetone Phosphate (DHAP) in Human Red Blood Cells by HPLC-TripleTOF 5600™ Mass Spectrometer
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    Chapter 11 Simultaneous Quantitation of Estradiol and Estrone in Serum Using Liquid Chromatography Mass Spectrometry
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    Chapter 12 Direct Measurement of Free Estradiol in Human Serum and Plasma by Equilibrium Dialysis-Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 13 Quantification of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 14 Quantitation of Insulin Analogues in Serum Using Immunoaffinity Extraction, Liquid Chromatography, and Tandem Mass Spectrometry
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    Chapter 15 Quantitation of Insulin-Like Growth Factor 1 in Serum by Liquid Chromatography High Resolution Accurate-Mass Mass Spectrometry
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    Chapter 16 Quantitation of Free Metanephrines in Plasma by Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 17 Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 18 High-Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine by LC-MS/MS. Method for Analyzing Isomers Without Chromatographic Separation
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    Chapter 19 Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 20 Quantitative Organic Acids in Urine by Two Dimensional Gas Chromatography-Time of Flight Mass Spectrometry (GCxGC-TOFMS)
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    Chapter 21 High Sensitivity Measurement of Pancreatic Polypeptide and Its Variant in Serum and Plasma by LC-MS/MS
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    Chapter 22 Quantitation of Parathyroid Hormone in Serum or Plasma by Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 23 Determination of Phenylalanine and Tyrosine by High Performance Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 24 Urine Purine Metabolite Determination by UPLC-Tandem Mass Spectrometry.
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    Chapter 25 Urine Pyrimidine Metabolite Determination by HPLC Tandem Mass Spectrometry
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    Chapter 26 Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 27 Quantitation of S-Adenosylmethionine and S-Adenosylhomocysteine in Plasma Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 28 A Simple, High-Throughput Method for Analysis of Ceramide, Glucosylceramide, and Ceramide Trihexoside in Dried Blood Spots by LC/MS/MS
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    Chapter 29 Quantification of Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone by Liquid Chromatography-Tandem Mass Spectrometry (LC/MS/MS)
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    Chapter 30 Urinary Succinylacetone Analysis by Gas Chromatography-Mass Spectrometry (GC-MS)
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    Chapter 31 Quantification of 1,25-Dihydroxyvitamin D2 and D3 in Serum Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 32 High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation
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    Chapter 33 Quantitation of 25-OH-Vitamin-D 2 and 25-OH-Vitamin-D 3 in Urine Using LC-MS/MS
Attention for Chapter 26: Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
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Chapter title
Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
Chapter number 26
Book title
Clinical Applications of Mass Spectrometry in Biomolecular Analysis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3182-8_26
Pubmed ID
Book ISBNs
978-1-4939-3181-1, 978-1-4939-3182-8
Authors

J. Grace Van Der Gugten, Daniel T. Holmes

Abstract

Accurate determination of plasma renin activity (PRA) is essential for the development and maintenance of an effective screening program for primary aldosteronism (PA). PRA measurement can also be useful in the investigation of renal artery stenosis, syndrome of mineralocorticoid excess, Addison's disease, congenital adrenal hyperplasia, Bartter and Gitelman syndromes, and for inherited defects in the renin angiotensin aldosterone system (RAAS). We describe a semi-automated and simple method for the accurate and precise measurement of PRA from 500 μL of plasma (250 μL if blank subtraction is omitted, as discussed) using a liquid chromatography and tandem mass spectrometry (LC-MS/MS) method for angiotensin I (AngI) in 96-well format. After a 3 h AngI generation step at 37 °C in buffering conditions at pH 6, the reaction is quenched with 10 % formic acid containing AngI internal standard. Sample preparation then proceeds with offline solid phase extraction, two wash steps, and methanol elution followed by injection into the LC-MS/MS system. Quantitation is performed against a 7-point calibration linear curve prepared in buffer. The assay calibration range is 0.34-30.0 ng/mL which corresponds to PRA values of 0.11-10.0 ng/mL/h: much wider than was possible using traditional competitive antibody-based methods. Total precision in clinical production has been observed to be 5.8 to 5.0 % for Bio-Rad Hypertension Control materials having nominal PRA values ranging from 1.73 to 12.43 ng/mL/h. At AngI concentrations of 0.06 ng/L (corresponding to a PRA of 0.02 ng/mL/h), signal to noise ratios are 50:1 indicating that the limit of quantitation is well below the level required for clinical use.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 33%
Researcher 3 25%
Student > Master 2 17%
Librarian 1 8%
Student > Doctoral Student 1 8%
Other 1 8%
Readers by discipline Count As %
Medicine and Dentistry 4 33%
Biochemistry, Genetics and Molecular Biology 2 17%
Agricultural and Biological Sciences 2 17%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Social Sciences 1 8%
Other 1 8%
Unknown 1 8%