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Clinical Applications of Mass Spectrometry in Biomolecular Analysis

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Cover of 'Clinical Applications of Mass Spectrometry in Biomolecular Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Mass Spectrometry in Clinical Laboratory: Applications in Biomolecular Analysis
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    Chapter 2 Quantification of Free Carnitine and Acylcarnitines in Plasma or Serum Using HPLC/MS/MS
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    Chapter 3 Quantification of Arginine and Its Methylated Derivatives in Plasma by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 4 Quantitation of Albumin in Urine by Liquid Chromatography Tandem Mass Spectrometry
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    Chapter 5 Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 6 Quantification of Five Clinically Important Amino Acids by HPLC-Triple TOF™ 5600 Based on Pre-column Double Derivatization Method
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    Chapter 7 Sensitive, Simple, and Robust Nano-Liquid Chromatography-Mass Spectrometry Method for Amyloid Protein Subtyping
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    Chapter 8 Quantitation of Ubiquinone (Coenzyme Q 10 ) in Serum/Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (ESI-LC-MS/MS)
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    Chapter 9 Quantitative Analysis of Salivary Cortisol Using LC-MS/MS
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    Chapter 10 Quantification of Dihydroxyacetone Phosphate (DHAP) in Human Red Blood Cells by HPLC-TripleTOF 5600™ Mass Spectrometer
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    Chapter 11 Simultaneous Quantitation of Estradiol and Estrone in Serum Using Liquid Chromatography Mass Spectrometry
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    Chapter 12 Direct Measurement of Free Estradiol in Human Serum and Plasma by Equilibrium Dialysis-Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 13 Quantification of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 14 Quantitation of Insulin Analogues in Serum Using Immunoaffinity Extraction, Liquid Chromatography, and Tandem Mass Spectrometry
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    Chapter 15 Quantitation of Insulin-Like Growth Factor 1 in Serum by Liquid Chromatography High Resolution Accurate-Mass Mass Spectrometry
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    Chapter 16 Quantitation of Free Metanephrines in Plasma by Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 17 Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 18 High-Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine by LC-MS/MS. Method for Analyzing Isomers Without Chromatographic Separation
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    Chapter 19 Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 20 Quantitative Organic Acids in Urine by Two Dimensional Gas Chromatography-Time of Flight Mass Spectrometry (GCxGC-TOFMS)
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    Chapter 21 High Sensitivity Measurement of Pancreatic Polypeptide and Its Variant in Serum and Plasma by LC-MS/MS
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    Chapter 22 Quantitation of Parathyroid Hormone in Serum or Plasma by Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 23 Determination of Phenylalanine and Tyrosine by High Performance Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 24 Urine Purine Metabolite Determination by UPLC-Tandem Mass Spectrometry.
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    Chapter 25 Urine Pyrimidine Metabolite Determination by HPLC Tandem Mass Spectrometry
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    Chapter 26 Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 27 Quantitation of S-Adenosylmethionine and S-Adenosylhomocysteine in Plasma Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 28 A Simple, High-Throughput Method for Analysis of Ceramide, Glucosylceramide, and Ceramide Trihexoside in Dried Blood Spots by LC/MS/MS
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    Chapter 29 Quantification of Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone by Liquid Chromatography-Tandem Mass Spectrometry (LC/MS/MS)
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    Chapter 30 Urinary Succinylacetone Analysis by Gas Chromatography-Mass Spectrometry (GC-MS)
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    Chapter 31 Quantification of 1,25-Dihydroxyvitamin D2 and D3 in Serum Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 32 High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation
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    Chapter 33 Quantitation of 25-OH-Vitamin-D 2 and 25-OH-Vitamin-D 3 in Urine Using LC-MS/MS
Attention for Chapter 17: Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry
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Chapter title
Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry
Chapter number 17
Book title
Clinical Applications of Mass Spectrometry in Biomolecular Analysis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3182-8_17
Pubmed ID
Book ISBNs
978-1-4939-3181-1, 978-1-4939-3182-8
Authors

Jessica Gabler, Sihe Wang

Abstract

Measuring urinary metanephrines aides in the diagnosis of pheochromocytomas-catecholamine producing tumors. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) allows for greater sensitivity and simpler sample preparation as compared with other techniques. Here we describe a simple LC-MS/MS method for measuring metanephrines in urine. Each urine sample was treated with diphenylboronic acid to create boronate complexes, and then applied to a Bond-Elut Plexa cartridge. After solid phase extraction, samples were concentrated and analyzed on an Atlantis T3 column with chromatographic run time totaling 8.5 min. MS/MS was set in positive electrospray ionization mode with multiple reaction monitoring for data collection. The assay was linear from 0.2 to 27.4 μmol/L and 0.3 to 14.6 μmol/L for metanephrine and normetanephrine, respectively. Intra-assay and total precision at three concentration levels over 10 days were <5 % for metanephrine and <10 % for normetanephrine.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 20%
Student > Bachelor 1 20%
Other 1 20%
Student > Master 1 20%
Unknown 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 20%
Agricultural and Biological Sciences 1 20%
Economics, Econometrics and Finance 1 20%
Medicine and Dentistry 1 20%
Unknown 1 20%