↓ Skip to main content

Clinical Applications of Mass Spectrometry in Biomolecular Analysis

Overview of attention for book
Cover of 'Clinical Applications of Mass Spectrometry in Biomolecular Analysis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Mass Spectrometry in Clinical Laboratory: Applications in Biomolecular Analysis
  3. Altmetric Badge
    Chapter 2 Quantification of Free Carnitine and Acylcarnitines in Plasma or Serum Using HPLC/MS/MS
  4. Altmetric Badge
    Chapter 3 Quantification of Arginine and Its Methylated Derivatives in Plasma by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
  5. Altmetric Badge
    Chapter 4 Quantitation of Albumin in Urine by Liquid Chromatography Tandem Mass Spectrometry
  6. Altmetric Badge
    Chapter 5 Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
  7. Altmetric Badge
    Chapter 6 Quantification of Five Clinically Important Amino Acids by HPLC-Triple TOF™ 5600 Based on Pre-column Double Derivatization Method
  8. Altmetric Badge
    Chapter 7 Sensitive, Simple, and Robust Nano-Liquid Chromatography-Mass Spectrometry Method for Amyloid Protein Subtyping
  9. Altmetric Badge
    Chapter 8 Quantitation of Ubiquinone (Coenzyme Q 10 ) in Serum/Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (ESI-LC-MS/MS)
  10. Altmetric Badge
    Chapter 9 Quantitative Analysis of Salivary Cortisol Using LC-MS/MS
  11. Altmetric Badge
    Chapter 10 Quantification of Dihydroxyacetone Phosphate (DHAP) in Human Red Blood Cells by HPLC-TripleTOF 5600™ Mass Spectrometer
  12. Altmetric Badge
    Chapter 11 Simultaneous Quantitation of Estradiol and Estrone in Serum Using Liquid Chromatography Mass Spectrometry
  13. Altmetric Badge
    Chapter 12 Direct Measurement of Free Estradiol in Human Serum and Plasma by Equilibrium Dialysis-Liquid Chromatography-Tandem Mass Spectrometry
  14. Altmetric Badge
    Chapter 13 Quantification of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
  15. Altmetric Badge
    Chapter 14 Quantitation of Insulin Analogues in Serum Using Immunoaffinity Extraction, Liquid Chromatography, and Tandem Mass Spectrometry
  16. Altmetric Badge
    Chapter 15 Quantitation of Insulin-Like Growth Factor 1 in Serum by Liquid Chromatography High Resolution Accurate-Mass Mass Spectrometry
  17. Altmetric Badge
    Chapter 16 Quantitation of Free Metanephrines in Plasma by Liquid Chromatography-Tandem Mass Spectrometry
  18. Altmetric Badge
    Chapter 17 Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry
  19. Altmetric Badge
    Chapter 18 High-Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine by LC-MS/MS. Method for Analyzing Isomers Without Chromatographic Separation
  20. Altmetric Badge
    Chapter 19 Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
  21. Altmetric Badge
    Chapter 20 Quantitative Organic Acids in Urine by Two Dimensional Gas Chromatography-Time of Flight Mass Spectrometry (GCxGC-TOFMS)
  22. Altmetric Badge
    Chapter 21 High Sensitivity Measurement of Pancreatic Polypeptide and Its Variant in Serum and Plasma by LC-MS/MS
  23. Altmetric Badge
    Chapter 22 Quantitation of Parathyroid Hormone in Serum or Plasma by Liquid Chromatography-Tandem Mass Spectrometry
  24. Altmetric Badge
    Chapter 23 Determination of Phenylalanine and Tyrosine by High Performance Liquid Chromatography-Tandem Mass Spectrometry
  25. Altmetric Badge
    Chapter 24 Urine Purine Metabolite Determination by UPLC-Tandem Mass Spectrometry.
  26. Altmetric Badge
    Chapter 25 Urine Pyrimidine Metabolite Determination by HPLC Tandem Mass Spectrometry
  27. Altmetric Badge
    Chapter 26 Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
  28. Altmetric Badge
    Chapter 27 Quantitation of S-Adenosylmethionine and S-Adenosylhomocysteine in Plasma Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
  29. Altmetric Badge
    Chapter 28 A Simple, High-Throughput Method for Analysis of Ceramide, Glucosylceramide, and Ceramide Trihexoside in Dried Blood Spots by LC/MS/MS
  30. Altmetric Badge
    Chapter 29 Quantification of Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone by Liquid Chromatography-Tandem Mass Spectrometry (LC/MS/MS)
  31. Altmetric Badge
    Chapter 30 Urinary Succinylacetone Analysis by Gas Chromatography-Mass Spectrometry (GC-MS)
  32. Altmetric Badge
    Chapter 31 Quantification of 1,25-Dihydroxyvitamin D2 and D3 in Serum Using Liquid Chromatography-Tandem Mass Spectrometry
  33. Altmetric Badge
    Chapter 32 High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation
  34. Altmetric Badge
    Chapter 33 Quantitation of 25-OH-Vitamin-D 2 and 25-OH-Vitamin-D 3 in Urine Using LC-MS/MS
Attention for Chapter 17: Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry
Altmetric Badge

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
2 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry
Chapter number 17
Book title
Clinical Applications of Mass Spectrometry in Biomolecular Analysis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3182-8_17
Pubmed ID
Book ISBNs
978-1-4939-3181-1, 978-1-4939-3182-8
Authors

Jessica Gabler, Sihe Wang

Abstract

Measuring urinary metanephrines aides in the diagnosis of pheochromocytomas-catecholamine producing tumors. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) allows for greater sensitivity and simpler sample preparation as compared with other techniques. Here we describe a simple LC-MS/MS method for measuring metanephrines in urine. Each urine sample was treated with diphenylboronic acid to create boronate complexes, and then applied to a Bond-Elut Plexa cartridge. After solid phase extraction, samples were concentrated and analyzed on an Atlantis T3 column with chromatographic run time totaling 8.5 min. MS/MS was set in positive electrospray ionization mode with multiple reaction monitoring for data collection. The assay was linear from 0.2 to 27.4 μmol/L and 0.3 to 14.6 μmol/L for metanephrine and normetanephrine, respectively. Intra-assay and total precision at three concentration levels over 10 days were <5 % for metanephrine and <10 % for normetanephrine.

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 50%
Student > Master 1 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 50%
Medicine and Dentistry 1 50%