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Clinical Applications of Mass Spectrometry in Biomolecular Analysis

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Cover of 'Clinical Applications of Mass Spectrometry in Biomolecular Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Mass Spectrometry in Clinical Laboratory: Applications in Biomolecular Analysis
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    Chapter 2 Quantification of Free Carnitine and Acylcarnitines in Plasma or Serum Using HPLC/MS/MS
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    Chapter 3 Quantification of Arginine and Its Methylated Derivatives in Plasma by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 4 Quantitation of Albumin in Urine by Liquid Chromatography Tandem Mass Spectrometry
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    Chapter 5 Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 6 Quantification of Five Clinically Important Amino Acids by HPLC-Triple TOF™ 5600 Based on Pre-column Double Derivatization Method
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    Chapter 7 Sensitive, Simple, and Robust Nano-Liquid Chromatography-Mass Spectrometry Method for Amyloid Protein Subtyping
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    Chapter 8 Quantitation of Ubiquinone (Coenzyme Q 10 ) in Serum/Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (ESI-LC-MS/MS)
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    Chapter 9 Quantitative Analysis of Salivary Cortisol Using LC-MS/MS
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    Chapter 10 Quantification of Dihydroxyacetone Phosphate (DHAP) in Human Red Blood Cells by HPLC-TripleTOF 5600™ Mass Spectrometer
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    Chapter 11 Simultaneous Quantitation of Estradiol and Estrone in Serum Using Liquid Chromatography Mass Spectrometry
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    Chapter 12 Direct Measurement of Free Estradiol in Human Serum and Plasma by Equilibrium Dialysis-Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 13 Quantification of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 14 Quantitation of Insulin Analogues in Serum Using Immunoaffinity Extraction, Liquid Chromatography, and Tandem Mass Spectrometry
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    Chapter 15 Quantitation of Insulin-Like Growth Factor 1 in Serum by Liquid Chromatography High Resolution Accurate-Mass Mass Spectrometry
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    Chapter 16 Quantitation of Free Metanephrines in Plasma by Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 17 Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 18 High-Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine by LC-MS/MS. Method for Analyzing Isomers Without Chromatographic Separation
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    Chapter 19 Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 20 Quantitative Organic Acids in Urine by Two Dimensional Gas Chromatography-Time of Flight Mass Spectrometry (GCxGC-TOFMS)
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    Chapter 21 High Sensitivity Measurement of Pancreatic Polypeptide and Its Variant in Serum and Plasma by LC-MS/MS
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    Chapter 22 Quantitation of Parathyroid Hormone in Serum or Plasma by Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 23 Determination of Phenylalanine and Tyrosine by High Performance Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 24 Urine Purine Metabolite Determination by UPLC-Tandem Mass Spectrometry.
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    Chapter 25 Urine Pyrimidine Metabolite Determination by HPLC Tandem Mass Spectrometry
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    Chapter 26 Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 27 Quantitation of S-Adenosylmethionine and S-Adenosylhomocysteine in Plasma Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 28 A Simple, High-Throughput Method for Analysis of Ceramide, Glucosylceramide, and Ceramide Trihexoside in Dried Blood Spots by LC/MS/MS
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    Chapter 29 Quantification of Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone by Liquid Chromatography-Tandem Mass Spectrometry (LC/MS/MS)
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    Chapter 30 Urinary Succinylacetone Analysis by Gas Chromatography-Mass Spectrometry (GC-MS)
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    Chapter 31 Quantification of 1,25-Dihydroxyvitamin D2 and D3 in Serum Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 32 High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation
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    Chapter 33 Quantitation of 25-OH-Vitamin-D 2 and 25-OH-Vitamin-D 3 in Urine Using LC-MS/MS
Attention for Chapter 32: High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation
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Chapter title
High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation
Chapter number 32
Book title
Clinical Applications of Mass Spectrometry in Biomolecular Analysis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3182-8_32
Pubmed ID
Book ISBNs
978-1-4939-3181-1, 978-1-4939-3182-8
Authors

Judy Stone

Abstract

Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 % formic acid in acetonitrile, is added and mixed with a 96-channel ALH. After a 4-min delay for larger precipitates to settle to the bottom of the plate, the upper 36 % of the precipitate/supernatant mix is transferred with the 96-channel ALH to a Sigma Hybrid SPE(®) plate and vacuumed through for removal of phospholipids and precipitated proteins. The filtrate is collected in a second 96-well plate (collection plate) which is foil-sealed, placed in the autosampler (ALS), and injected into a multiplexed LC-MS/MS system running AB Sciex Cliquid(®) and MPX(®) software. Two Shimadzu LC stacks, with multiplex timing controlled by MPX(®) software, inject alternately to one AB Sciex API-5000 MS/MS using positive atmospheric pressure chemical ionization (APCI) and a 1.87 min water/acetonitrile LC gradient with a 2.1 × 20 mm, 2.7 μm, C18 fused core particle column (Sigma Ascentis Express). LC-MS/MS through put is ~44 samples/h/LC-MS/MS system with dual-LC channel multiplexing. Plate maps are transferred electronically from the ALH and reformatted into LC-MS/MS sample table format using the Data Innovations LLC (DI) Instrument Manager middleware application. Before collection plates are loaded into the ALS, the plate bar code is manually scanned to download the sample table from the DI middleware to the LC-MS/MS. After acquisition-LC-MS/MS data is analyzed with AB Sciex Multiquant(®) software using customized queries, and then results are transferred electronically via a DI interface to the LIS. 2500 samples/day can be extracted by two analysts using four ALHs in 4-6 h. LC-MS/MS analysis of those samples on three dual-channel LC multiplexed LC-MS/MS systems requires 19-21 h and data analysis can be done by two analysts in 4-6 h.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Student > Doctoral Student 1 33%
Lecturer > Senior Lecturer 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Immunology and Microbiology 1 33%
Chemistry 1 33%