↓ Skip to main content

Fluorescent Protein-Based Biosensors

Overview of attention for book
Cover of 'Fluorescent Protein-Based Biosensors'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Design and Application of Genetically Encodable Biosensors Based on Fluorescent Proteins
  3. Altmetric Badge
    Chapter 2 An Introduction to Fluorescence Imaging Techniques Geared Towards Biosensor Applications
  4. Altmetric Badge
    Chapter 3 Fluorescent Protein-Based Biosensors
  5. Altmetric Badge
    Chapter 4 Detecting cAMP with an Epac-Based FRET Sensor in Single Living Cells
  6. Altmetric Badge
    Chapter 5 Analysis of Compartmentalized cAMP: A Method to Compare Signals from Differently Targeted FRET Reporters
  7. Altmetric Badge
    Chapter 6 Genetically Encoded Fluorescent Biosensors for Live Cell Imaging of Lipid Dynamics
  8. Altmetric Badge
    Chapter 7 Live-Cell Imaging of Cytosolic NADH–NAD + Redox State Using a Genetically Encoded Fluorescent Biosensor
  9. Altmetric Badge
    Chapter 8 Measuring Membrane Voltage with Microbial Rhodopsins
  10. Altmetric Badge
    Chapter 9 Imaging the Activity of Ras Superfamily GTPase Proteins in Small Subcellular Compartments in Neurons
  11. Altmetric Badge
    Chapter 10 Imaging Kinase Activity at Protein Scaffolds
  12. Altmetric Badge
    Chapter 11 Using a Genetically Encoded FRET-Based Reporter to Visualize Calcineurin Phosphatase Activity in Living Cells
  13. Altmetric Badge
    Chapter 12 Genetically Encoded FRET Indicators for Live-Cell Imaging of Histone Acetylation.
  14. Altmetric Badge
    Chapter 13 Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity.
  15. Altmetric Badge
    Chapter 14 Biosensor imaging in brain slice preparations.
  16. Altmetric Badge
    Chapter 15 Optical Calcium Imaging Using DNA-Encoded Fluorescence Sensors in Transgenic Fruit Flies, Drosophila melanogaster.
  17. Altmetric Badge
    Chapter 16 A Multiparameter Live Cell Imaging Approach to Monitor Cyclic AMP and Protein Kinase A Dynamics in Parallel
  18. Altmetric Badge
    Chapter 17 FRET and BRET-Based Biosensors in Live Cell Compound Screens.
  19. Altmetric Badge
    Chapter 18 Integrating fluorescent biosensor data using computational models.
Attention for Chapter 15: Optical Calcium Imaging Using DNA-Encoded Fluorescence Sensors in Transgenic Fruit Flies, Drosophila melanogaster.
Altmetric Badge

Mentioned by

twitter
1 tweeter

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
12 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Optical Calcium Imaging Using DNA-Encoded Fluorescence Sensors in Transgenic Fruit Flies, Drosophila melanogaster.
Chapter number 15
Book title
Fluorescent Protein-Based Biosensors
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-622-1_15
Pubmed ID
Book ISBNs
978-1-62703-621-4, 978-1-62703-622-1
Authors

Shubham Dipt, Thomas Riemensperger, André Fiala

Abstract

The invention of protein-based fluorescent biosensors has paved the way to target specific cells with these probes and visualize intracellular processes not only in isolated cells or tissue cultures but also in transgenic animals. In particular, DNA-encoded fluorescence proteins sensitive to Ca(2+) ions are often used to monitor changes in intracellular Ca(2+) concentrations. This is of particular relevance in neuroscience since the dynamics of intracellular Ca(2+) concentrations represents a faithful correlate for neuronal activity, and optical Ca(2+) imaging is commonly used to monitor spatiotemporal activity across populations of neurons. In this respect Drosophila provides a favorable model organism due to the sophisticated genetic tools that facilitate the targeted expression of fluorescent Ca(2+) sensor proteins. Here we describe how optical Ca(2+) imaging of neuronal activity in the Drosophila brain can be carried out in vivo using two-photon microscopy. We exemplify this technique by describing how to monitor odor-evoked Ca(2+) dynamics in the primary olfactory center of the Drosophila brain.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 3 25%
Student > Master 2 17%
Student > Doctoral Student 2 17%
Researcher 1 8%
Professor > Associate Professor 1 8%
Other 1 8%
Unknown 2 17%
Readers by discipline Count As %
Neuroscience 6 50%
Agricultural and Biological Sciences 4 33%
Unknown 2 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2013.
All research outputs
#2,902,132
of 3,628,758 outputs
Outputs from Methods in molecular biology
#1,439
of 2,357 outputs
Outputs of similar age
#73,198
of 92,169 outputs
Outputs of similar age from Methods in molecular biology
#24
of 47 outputs
Altmetric has tracked 3,628,758 research outputs across all sources so far. This one is in the 2nd percentile – i.e., 2% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,357 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 92,169 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.