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Functional Analysis of DNA and Chromatin

Overview of attention for book
Cover of 'Functional Analysis of DNA and Chromatin'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern
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    Chapter 2 Using Microchip Gel Electrophoresis to Probe DNA–Drug Binding Interactions
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    Chapter 3 Identifying Different Types of Chromatin Using Giemsa Staining
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    Chapter 4 Analysis of DNA Damage and Repair by Comet Fluorescence In Situ Hybridization (Comet-FISH)
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    Chapter 5 Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level
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    Chapter 6 Polarization Microscopy of Extended Chromatin Fibers
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    Chapter 7 DNA Labeling In Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image Processing Software
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    Chapter 8 A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation
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    Chapter 9 Image Analysis of Chromatin Remodelling
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    Chapter 10 FISH Methods in Cytogenetic Studies
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    Chapter 11 Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker
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    Chapter 12 Atomic Force Microscopy for Analyzing Metaphase Chromosomes: Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns
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    Chapter 13 Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections
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    Chapter 14 Selective Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage Studies
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    Chapter 15 Visualization and Interpretation of Eukaryotic DNA Replication Intermediates In Vivo by Electron Microscopy
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    Chapter 16 Combined Bidimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replication Intermediates in Human Cells
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    Chapter 17 Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine (5mC) Immunological Detection
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    Chapter 18 Methyl-Combing: Single-Molecule Analysis of DNA Methylation on Stretched DNA Fibers
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    Chapter 19 Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art
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    Chapter 20 Hydroxymethylated DNA Immunoprecipitation (hmeDIP)
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    Chapter 21 Microscale thermophoresis for the assessment of nuclear protein-binding affinities.
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    Chapter 22 Analysis of Histone Posttranslational Modifications from Nucleolus-Associated Chromatin by Mass Spectrometry
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    Chapter 23 Salt-urea, sulfopropyl-sepharose, and covalent chromatography methods for histone isolation and fractionation.
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    Chapter 24 Chromatin Immunoprecipitation
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    Chapter 25 Analysis of Chromatin Composition of Repetitive Sequences: The ChIP-Chop Assay
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    Chapter 26 Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae
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    Chapter 27 Deep Sequencing of Small Chromatin-Associated RNA: Isolation and Library Preparation.
  29. Altmetric Badge
    Chapter 28 Deep Sequencing of Small Chromatin-Associated RNA: Bioinformatic Analysis.
Attention for Chapter 20: Hydroxymethylated DNA Immunoprecipitation (hmeDIP)
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (59th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

Mentioned by

3 tweeters


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Chapter title
Hydroxymethylated DNA Immunoprecipitation (hmeDIP)
Chapter number 20
Book title
Functional Analysis of DNA and Chromatin
Published in
Methods in molecular biology, October 2013
DOI 10.1007/978-1-62703-706-8_20
Pubmed ID
Book ISBNs
978-1-62703-705-1, 978-1-62703-706-8

Colm E. Nestor, Richard R. Meehan, Nestor CE, Meehan RR, Nestor, Colm E., Meehan, Richard R.


5-hydroxymethylcytosine (5hmC) was recently identified as an abundant epigenetic mark in mammals. Subsequent research has implicated 5hmC in normal mammalian development and disease pathogenesis in humans. Many of the techniques commonly used to assay for canonical 5-methylcytosine (5mC) cannot distinguish between 5hmC and 5mC. The development of antibodies specific to 5hmC has allowed for specific enrichment of DNA fragments containing 5hmC. Hydroxymethylated DNA immunoprecipitation (hmeDIP) has become an invaluable tool for determining both locus-specific and genome-wide profiles of 5hmC in mammalian DNA. Here, we describe the use of hmeDIP to characterize the relative abundance of 5hmC at loci in mammalian DNA.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Switzerland 1 3%
Canada 1 3%
Unknown 38 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 33%
Researcher 7 18%
Student > Bachelor 4 10%
Professor > Associate Professor 3 8%
Professor 3 8%
Other 6 15%
Unknown 4 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 40%
Biochemistry, Genetics and Molecular Biology 11 28%
Immunology and Microbiology 2 5%
Business, Management and Accounting 2 5%
Medicine and Dentistry 2 5%
Other 3 8%
Unknown 4 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 August 2014.
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Altmetric has tracked 9,703,212 research outputs across all sources so far. This one has received more attention than most of these and is in the 50th percentile.
So far Altmetric has tracked 7,426 research outputs from this source. They receive a mean Attention Score of 2.0. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 151,912 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.