Chapter title |
Identifying Different Types of Chromatin Using Giemsa Staining
|
---|---|
Chapter number | 3 |
Book title |
Functional Analysis of DNA and Chromatin
|
Published in |
Methods in molecular biology, October 2013
|
DOI | 10.1007/978-1-62703-706-8_3 |
Pubmed ID | |
Book ISBNs |
978-1-62703-705-1, 978-1-62703-706-8
|
Authors |
Stockert, Juan C., Blázquez-Castro, Alfonso, Horobin, Richard W., Juan C. Stockert, Alfonso Blázquez-Castro, Richard W. Horobin |
Abstract |
Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli. After specific prestaining treatments that induce chromatin disorganization (giving banded or harlequin chromosomes), Giemsa staining produces a differential coloration, with C- and G-bands appearing in purple whereas remaining chromosome regions are blue. Unsubstituted (TT) and bromo-substituted (BT) DNAs also appear purple and blue, respectively. The same occurs in the case of BT and BB chromatids.In addition to discussing the use of Giemsa stain as a suitable method to reveal specific features of chromosome structure, some molecular processes and models are also described to explain Giemsa staining mechanisms of chromatin. |
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