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Functional Analysis of DNA and Chromatin

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Cover of 'Functional Analysis of DNA and Chromatin'

Table of Contents

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    Book Overview
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    Chapter 1 Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern
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    Chapter 2 Using Microchip Gel Electrophoresis to Probe DNA–Drug Binding Interactions
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    Chapter 3 Identifying Different Types of Chromatin Using Giemsa Staining
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    Chapter 4 Analysis of DNA Damage and Repair by Comet Fluorescence In Situ Hybridization (Comet-FISH)
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    Chapter 5 Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level
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    Chapter 6 Polarization Microscopy of Extended Chromatin Fibers
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    Chapter 7 DNA Labeling In Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image Processing Software
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    Chapter 8 A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation
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    Chapter 9 Image Analysis of Chromatin Remodelling
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    Chapter 10 FISH Methods in Cytogenetic Studies
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    Chapter 11 Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker
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    Chapter 12 Atomic Force Microscopy for Analyzing Metaphase Chromosomes: Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns
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    Chapter 13 Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections
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    Chapter 14 Selective Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage Studies
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    Chapter 15 Visualization and Interpretation of Eukaryotic DNA Replication Intermediates In Vivo by Electron Microscopy
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    Chapter 16 Combined Bidimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replication Intermediates in Human Cells
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    Chapter 17 Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine (5mC) Immunological Detection
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    Chapter 18 Methyl-Combing: Single-Molecule Analysis of DNA Methylation on Stretched DNA Fibers
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    Chapter 19 Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art
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    Chapter 20 Hydroxymethylated DNA Immunoprecipitation (hmeDIP)
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    Chapter 21 Microscale thermophoresis for the assessment of nuclear protein-binding affinities.
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    Chapter 22 Analysis of Histone Posttranslational Modifications from Nucleolus-Associated Chromatin by Mass Spectrometry
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    Chapter 23 Salt-urea, sulfopropyl-sepharose, and covalent chromatography methods for histone isolation and fractionation.
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    Chapter 24 Chromatin Immunoprecipitation
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    Chapter 25 Analysis of Chromatin Composition of Repetitive Sequences: The ChIP-Chop Assay
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    Chapter 26 Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae
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    Chapter 27 Deep Sequencing of Small Chromatin-Associated RNA: Isolation and Library Preparation.
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    Chapter 28 Deep Sequencing of Small Chromatin-Associated RNA: Bioinformatic Analysis.
Attention for Chapter 3: Identifying Different Types of Chromatin Using Giemsa Staining
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Chapter title
Identifying Different Types of Chromatin Using Giemsa Staining
Chapter number 3
Book title
Functional Analysis of DNA and Chromatin
Published in
Methods in molecular biology, October 2013
DOI 10.1007/978-1-62703-706-8_3
Pubmed ID
Book ISBNs
978-1-62703-705-1, 978-1-62703-706-8

Stockert, Juan C., Blázquez-Castro, Alfonso, Horobin, Richard W., Juan C. Stockert, Alfonso Blázquez-Castro, Richard W. Horobin


Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli. After specific prestaining treatments that induce chromatin disorganization (giving banded or harlequin chromosomes), Giemsa staining produces a differential coloration, with C- and G-bands appearing in purple whereas remaining chromosome regions are blue. Unsubstituted (TT) and bromo-substituted (BT) DNAs also appear purple and blue, respectively. The same occurs in the case of BT and BB chromatids.In addition to discussing the use of Giemsa stain as a suitable method to reveal specific features of chromosome structure, some molecular processes and models are also described to explain Giemsa staining mechanisms of chromatin.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Russia 1 6%
Unknown 16 94%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 29%
Researcher 5 29%
Student > Master 2 12%
Student > Ph. D. Student 1 6%
Professor > Associate Professor 1 6%
Other 1 6%
Unknown 2 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 47%
Agricultural and Biological Sciences 3 18%
Medicine and Dentistry 3 18%
Unknown 3 18%

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