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Functional Analysis of DNA and Chromatin

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Cover of 'Functional Analysis of DNA and Chromatin'

Table of Contents

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    Book Overview
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    Chapter 1 Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern
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    Chapter 2 Using Microchip Gel Electrophoresis to Probe DNA–Drug Binding Interactions
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    Chapter 3 Identifying Different Types of Chromatin Using Giemsa Staining
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    Chapter 4 Analysis of DNA Damage and Repair by Comet Fluorescence In Situ Hybridization (Comet-FISH)
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    Chapter 5 Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level
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    Chapter 6 Polarization Microscopy of Extended Chromatin Fibers
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    Chapter 7 DNA Labeling In Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image Processing Software
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    Chapter 8 A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation
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    Chapter 9 Image Analysis of Chromatin Remodelling
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    Chapter 10 FISH Methods in Cytogenetic Studies
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    Chapter 11 Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker
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    Chapter 12 Atomic Force Microscopy for Analyzing Metaphase Chromosomes: Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns
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    Chapter 13 Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections
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    Chapter 14 Selective Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage Studies
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    Chapter 15 Visualization and Interpretation of Eukaryotic DNA Replication Intermediates In Vivo by Electron Microscopy
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    Chapter 16 Combined Bidimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replication Intermediates in Human Cells
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    Chapter 17 Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine (5mC) Immunological Detection
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    Chapter 18 Methyl-Combing: Single-Molecule Analysis of DNA Methylation on Stretched DNA Fibers
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    Chapter 19 Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art
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    Chapter 20 Hydroxymethylated DNA Immunoprecipitation (hmeDIP)
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    Chapter 21 Microscale thermophoresis for the assessment of nuclear protein-binding affinities.
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    Chapter 22 Analysis of Histone Posttranslational Modifications from Nucleolus-Associated Chromatin by Mass Spectrometry
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    Chapter 23 Salt-urea, sulfopropyl-sepharose, and covalent chromatography methods for histone isolation and fractionation.
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    Chapter 24 Chromatin Immunoprecipitation
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    Chapter 25 Analysis of Chromatin Composition of Repetitive Sequences: The ChIP-Chop Assay
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    Chapter 26 Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae
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    Chapter 27 Deep Sequencing of Small Chromatin-Associated RNA: Isolation and Library Preparation.
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    Chapter 28 Deep Sequencing of Small Chromatin-Associated RNA: Bioinformatic Analysis.
Attention for Chapter 10: FISH Methods in Cytogenetic Studies
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