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Two Hybrid Technologies

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Cover of 'Two Hybrid Technologies'

Table of Contents

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    Book Overview
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    Chapter 1 Matrix-Based Yeast Two-Hybrid Screen Strategies and Comparison of Systems
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    Chapter 2 Array-based yeast two-hybrid screens: a practical guide.
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    Chapter 3 High-Throughput Yeast Two-Hybrid Screening
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    Chapter 4 A Stringent Yeast Two-Hybrid Matrix Screening Approach for Protein–Protein Interaction Discovery
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    Chapter 5 High-Throughput Yeast Two-Hybrid Screening of Complex cDNA Libraries
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    Chapter 6 Virus–Human Cell Interactomes
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    Chapter 7 Interactome Mapping in Malaria Parasites: Challenges and Opportunities
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    Chapter 8 Mapping Interactomes with High Coverage and Efficiency Using the Shifted Transversal Design
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    Chapter 9 Assigning Confidence Scores to Protein–Protein Interactions
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    Chapter 10 The Integration and Annotation of the Human Interactome in the UniHI Database
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    Chapter 11 Gene-Centered Yeast One-Hybrid Assays
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    Chapter 12 One- Plus Two-Hybrid System for the Efficient Selection of Missense Mutant Alleles Defective in Protein–Protein Interactions
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    Chapter 13 Investigation of Membrane Protein Interactions Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System
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    Chapter 14 Application of the Split-Protein Sensor Trp1 to Protein Interaction Discovery in the Yeast Saccharomyces cerevisiae
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    Chapter 15 Tetracycline Repressor-Based Mammalian Two-Hybrid Systems
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    Chapter 16 The Fluorescent Two-Hybrid (F2H) Assay for Direct Analysis of Protein–Protein Interactions in Living Cells
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    Chapter 17 ArrayMAPPIT: A Screening Platform for Human Protein Interactome Analysis
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    Chapter 18 MAPPIT as a High-Throughput Screening Assay for Modulators of Protein–Protein Interactions in HIV and HCV
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    Chapter 19 Integrated Measurement of Split TEV and Cis -Regulatory Assays Using EXT Encoded Reporter Libraries
Attention for Chapter 2: Array-based yeast two-hybrid screens: a practical guide.
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Chapter title
Array-based yeast two-hybrid screens: a practical guide.
Chapter number 2
Book title
Two Hybrid Technologies
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-455-1_2
Pubmed ID
Book ISBNs
978-1-61779-454-4, 978-1-61779-455-1

Roman Häuser, Thorsten Stellberger, Seesandra V. Rajagopala, Peter Uetz


Yeast two-hybrid screens are carried out as random library screens or matrix-based screens. The latter have the advantage of being better controlled and thus typically give clearer results. In this chapter, we provide detailed protocols for matrix-based Y2H screens and give some helpful instructions how to plan a large-scale interaction screen. We also discuss strategies to identify or avoid false negatives and false positives.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Singapore 1 7%
Unknown 14 93%

Demographic breakdown

Readers by professional status Count As %
Other 3 20%
Researcher 3 20%
Student > Ph. D. Student 3 20%
Professor > Associate Professor 2 13%
Student > Bachelor 1 7%
Other 1 7%
Unknown 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 60%
Biochemistry, Genetics and Molecular Biology 3 20%
Medicine and Dentistry 1 7%
Unknown 2 13%