Array-based yeast two-hybrid screens: a practical guide.
Two Hybrid Technologies
Methods in molecular biology, January 2012
Roman Häuser, Thorsten Stellberger, Seesandra V. Rajagopala, Peter Uetz
Yeast two-hybrid screens are carried out as random library screens or matrix-based screens. The latter have the advantage of being better controlled and thus typically give clearer results. In this chapter, we provide detailed protocols for matrix-based Y2H screens and give some helpful instructions how to plan a large-scale interaction screen. We also discuss strategies to identify or avoid false negatives and false positives.
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