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Plant Signal Transduction

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Cover of 'Plant Signal Transduction'

Table of Contents

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    Book Overview
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    Chapter 1 Suppressor Screens in Arabidopsis
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    Chapter 2 Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants.
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    Chapter 3 DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants.
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    Chapter 4 Identification of Genes Responsible for Natural Variation in Volatile Content Using Next-Generation Sequencing Technology.
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    Chapter 5 Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation
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    Chapter 6 Culture of the Tomato Micro-Tom Cultivar in Greenhouse
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    Chapter 7 Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry
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    Chapter 8 A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications
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    Chapter 9 Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function
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    Chapter 10 Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells
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    Chapter 11 Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins
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    Chapter 12 Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions.
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    Chapter 13 Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana
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    Chapter 14 Analysis of Protein–Lipid Interactions Using Purified C2 Domains
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    Chapter 15 Assessing Kinase Activity in Plants with In-Gel Kinase Assays
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    Chapter 16 Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins
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    Chapter 17 Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions.
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    Chapter 18 Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus
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    Chapter 19 Simultaneous Determination of Plant Hormones by GC-TOF-MS
Attention for Chapter 1: Suppressor Screens in Arabidopsis
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Chapter title
Suppressor Screens in Arabidopsis
Chapter number 1
Book title
Plant Signal Transduction
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3115-6_1
Pubmed ID
Book ISBNs
978-1-4939-3114-9, 978-1-4939-3115-6
Authors

Xin Li, Yuelin Zhang

Abstract

Genetic screens have proven to be a useful tool in the dissection of biological processes in plants. Specifically, suppressor screens have been widely used to study signal transduction pathways. Here we provide a detailed protocol for ethyl methanesulfonate (EMS) mutagenesis used in our suppressor screens in Arabidopsis and discuss the basic principles behind suppressor screen design and downstream analyses.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 58 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 24%
Student > Ph. D. Student 14 24%
Student > Bachelor 5 9%
Student > Master 5 9%
Student > Doctoral Student 4 7%
Other 4 7%
Unknown 12 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 28 48%
Biochemistry, Genetics and Molecular Biology 17 29%
Environmental Science 1 2%
Chemistry 1 2%
Engineering 1 2%
Other 0 0%
Unknown 10 17%