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Mendeley readers
| Chapter title |
Assessing Kinase Activity in Plants with In-Gel Kinase Assays
|
|---|---|
| Chapter number | 15 |
| Book title |
Plant Signal Transduction
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3115-6_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3114-9, 978-1-4939-3115-6
|
| Authors |
Pengcheng Wang, Jian-Kang Zhu, Wang, Pengcheng, Zhu, Jian-Kang |
| Abstract |
The in-gel protein kinase assay is a powerful method to measure the protein phosphorylation activity of specific protein kinases. Any protein substrate can be embedded in polyacrylamide gels where they can be phosphorylated by protein kinases that are separated in the gel under denaturing conditions and then renatured. The kinase activity can be visualized in situ in the gels by autoradiography. This method has been used to compare the activities of protein kinases in parallel samples or to identify their potential substrates. Here, we describe in detail an in-gel kinase assay to measure the activity of some protein kinases in plants. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 20% |
| Professor > Associate Professor | 2 | 10% |
| Student > Ph. D. Student | 2 | 10% |
| Unspecified | 1 | 5% |
| Student > Bachelor | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 9 | 45% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 35% |
| Agricultural and Biological Sciences | 3 | 15% |
| Unspecified | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Unknown | 8 | 40% |