| Chapter title |
Analysis of Protein–Lipid Interactions Using Purified C2 Domains
|
|---|---|
| Chapter number | 14 |
| Book title |
Plant Signal Transduction
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3115-6_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3114-9, 978-1-4939-3115-6
|
| Authors |
Jessica Pérez-Sancho, Arnaldo L. Schapire, Miguel A. Botella, Abel Rosado |
| Abstract |
C2 domains (C2s) are regulatory protein modules identified in eukaryotic proteins targeted to cell membranes. C2s were initially characterized as independently folded Ca(2+)-dependent phospholipids binding domains; however, later studies have shown that C2s have evolutionarily diverged into Ca(2+)-dependent and Ca(2+)-independent forms. These forms interact and regulate their affinity to diverse lipid species using different binding mechanisms. In this protocol we describe a biochemical approach to produce, purify, and solubilize functional C2 domains bound to GST for the identification of their putative Ca(2+)-dependent and Ca(2+)-independent lipid-binding partners. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 8% |
| Unknown | 12 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 15% |
| Student > Doctoral Student | 2 | 15% |
| Researcher | 2 | 15% |
| Student > Bachelor | 1 | 8% |
| Professor | 1 | 8% |
| Other | 4 | 31% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 62% |
| Biochemistry, Genetics and Molecular Biology | 3 | 23% |
| Chemistry | 1 | 8% |
| Unknown | 1 | 8% |