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Biotechnology of Plant Secondary Metabolism

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Cover of 'Biotechnology of Plant Secondary Metabolism'

Table of Contents

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    Book Overview
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    Chapter 1 Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal.
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    Chapter 2 Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction
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    Chapter 3 A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines
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    Chapter 4 Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
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    Chapter 5 From Plant Extract to a cDNA Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica
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    Chapter 6 Production of Recombinant Caffeine Synthase from Guarana ( Paullinia cupana var. sorbilis ) in Escherichia coli
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    Chapter 7 Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
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    Chapter 8 Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast.
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    Chapter 9 Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
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    Chapter 10 A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
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    Chapter 11 Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
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    Chapter 12 Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
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    Chapter 13 Protoplast Transformation as a Plant-Transferable Transient Expression System
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    Chapter 14 Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System.
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    Chapter 15 Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs.
Attention for Chapter 9: Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
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Chapter title
Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
Chapter number 9
Book title
Biotechnology of Plant Secondary Metabolism
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3393-8_9
Pubmed ID
Book ISBNs
978-1-4939-3391-4, 978-1-4939-3393-8
Authors

Fernanda de Costa, Carla J. S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, Patrick S. Covello

Abstract

Short peptide tags genetically fused to recombinant proteins have been widely used to facilitate detection or purification without the need to develop specific procedures. In general, an ideal affinity tag would allow the efficient purification of tagged proteins in high yield, without affecting its function. Here, we describe the purification steps to purify a recombinant polyhistidine-tagged glucosyltransferase from Centella asiatica using immobilized metal affinity chromatography.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 60%
Student > Master 2 20%
Student > Postgraduate 1 10%
Lecturer 1 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 80%
Agricultural and Biological Sciences 2 20%