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Biotechnology of Plant Secondary Metabolism

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Cover of 'Biotechnology of Plant Secondary Metabolism'

Table of Contents

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    Book Overview
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    Chapter 1 Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal.
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    Chapter 2 Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction
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    Chapter 3 A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines
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    Chapter 4 Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
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    Chapter 5 From Plant Extract to a cDNA Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica
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    Chapter 6 Production of Recombinant Caffeine Synthase from Guarana ( Paullinia cupana var. sorbilis ) in Escherichia coli
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    Chapter 7 Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
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    Chapter 8 Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast.
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    Chapter 9 Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
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    Chapter 10 A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
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    Chapter 11 Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
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    Chapter 12 Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
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    Chapter 13 Protoplast Transformation as a Plant-Transferable Transient Expression System
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    Chapter 14 Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System.
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    Chapter 15 Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs.
Attention for Chapter 7: Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
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Chapter title
Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
Chapter number 7
Book title
Biotechnology of Plant Secondary Metabolism
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3393-8_7
Pubmed ID
Book ISBNs
978-1-4939-3391-4, 978-1-4939-3393-8
Authors

Vishal Singh Negi, Dulal Borthakur, Negi, Vishal Singh, Borthakur, Dulal

Abstract

Heterologous expression of eukaryotic genes in bacterial system is an important method in synthetic biology to characterize proteins. It is a widely used method, which can be sometimes quite challenging, as a number of factors that act along the path of expression of a transgene to mRNA, and mRNA to protein, can potentially affect the expression of a transgene in a heterologous system. Here, we describe a method for successful cloning and expression of mimosinase-encoding gene from Leucaena leucocephala (leucaena) in E. coli as the heterologous host. Mimosinase is an important enzyme especially in the context of metabolic engineering of plant secondary metabolite as it catalyzes the degradation of mimosine, which is a toxic secondary metabolite found in all Leucaena and Mimosa species. We also describe the methods used for characterization of the recombinant mimosinase.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 50%
Professor > Associate Professor 1 17%
Student > Ph. D. Student 1 17%
Unknown 1 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 33%
Agricultural and Biological Sciences 2 33%
Nursing and Health Professions 1 17%
Unknown 1 17%