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Biotechnology of Plant Secondary Metabolism

Overview of attention for book
Cover of 'Biotechnology of Plant Secondary Metabolism'

Table of Contents

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    Book Overview
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    Chapter 1 Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal.
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    Chapter 2 Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction
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    Chapter 3 A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines
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    Chapter 4 Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
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    Chapter 5 From Plant Extract to a cDNA Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica
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    Chapter 6 Production of Recombinant Caffeine Synthase from Guarana ( Paullinia cupana var. sorbilis ) in Escherichia coli
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    Chapter 7 Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
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    Chapter 8 Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast.
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    Chapter 9 Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
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    Chapter 10 A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
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    Chapter 11 Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
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    Chapter 12 Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
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    Chapter 13 Protoplast Transformation as a Plant-Transferable Transient Expression System
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    Chapter 14 Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System.
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    Chapter 15 Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs.
Attention for Chapter 15: Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs.
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Chapter title
Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs.
Chapter number 15
Book title
Biotechnology of Plant Secondary Metabolism
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3393-8_15
Pubmed ID
Book ISBNs
978-1-4939-3391-4, 978-1-4939-3393-8
Authors

Chi, Ming, Bhagwat, Basdeo, Tang, Guiliang, Xiang, Yu, Ming Chi, Basdeo Bhagwat, Guiliang Tang, Yu Xiang

Abstract

It is of great importance and interest to develop crop varieties with low polyphenol oxidase (PPO) activity for the food industry because PPO-mediated oxidative browning is a main cause of post-harvest deterioration and quality loss of fresh produce and processed foods. We recently demonstrated that potato tubers with reduced browning phenotypes can be produced by inhibition of the expression of several PPO gene isoforms using artificial microRNA (amiRNA) technology. The approach introduces a single type of 21-nucleotide RNA population to guide silencing of the PPO gene transcripts in potato tissues. Some advantages of the technology are: small RNA molecules are genetically transformed, off-target gene silencing can be avoided or minimized at the stage of amiRNA designs, and accuracy and efficiency of the processes can be detected at every step using molecular biological techniques. Here we describe the methods for transformation and regeneration of potatoes with amiRNA vectors, detection of the expression of amiRNAs, identification of the cleaved product of the target gene transcripts, and assay of the expression level of PPO gene isoforms in potatoes.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 38%
Student > Bachelor 3 38%
Professor > Associate Professor 1 13%
Student > Ph. D. Student 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 75%
Biochemistry, Genetics and Molecular Biology 1 13%
Engineering 1 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2016.
All research outputs
#7,489,858
of 9,703,553 outputs
Outputs from Methods in molecular biology
#3,789
of 7,426 outputs
Outputs of similar age
#238,464
of 342,356 outputs
Outputs of similar age from Methods in molecular biology
#556
of 1,132 outputs
Altmetric has tracked 9,703,553 research outputs across all sources so far. This one is in the 12th percentile – i.e., 12% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,426 research outputs from this source. They receive a mean Attention Score of 2.0. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 342,356 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,132 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.