Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast.
Biotechnology of Plant Secondary Metabolism
Methods in molecular biology, January 2016
Emmerstorfer-Augustin, Anita, Pichler, Harald, Anita Emmerstorfer-Augustin, Harald Pichler
Plant terpenoids are high-value compounds broadly applied as food additives or fragrances in perfumes and cosmetics. Their biotechnological production in yeast offers an attractive alternative to extraction from plants. Here, we provide two optimized protocols for the production of the plant terpenoid trans-nootkatol with recombinant S. cerevisiae by either (I) converting externally added (+)-valencene with resting cells or (II) cultivating engineered self-sufficient production strains. By synthesis of the hydrophobic compounds in self-sufficient production cells, phase transfer issues can be avoided and the highly volatile products can be enriched in and easily purified from n-dodecane, which is added to the cell broth as second phase.
|Members of the public||1||100%|
|Readers by professional status||Count||As %|
|Student > Ph. D. Student||3||19%|
|Student > Doctoral Student||2||13%|
|Student > Master||2||13%|
|Professor > Associate Professor||1||6%|
|Readers by discipline||Count||As %|
|Agricultural and Biological Sciences||5||31%|
|Biochemistry, Genetics and Molecular Biology||4||25%|
|Medicine and Dentistry||1||6%|