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MicroRNA and Cancer

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Cover of 'MicroRNA and Cancer'

Table of Contents

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    Book Overview
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    Chapter 1 Noncoding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics
  3. Altmetric Badge
    Chapter 2 MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential
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    Chapter 3 Involvement of miRNAs and Pseudogenes in Cancer
  5. Altmetric Badge
    Chapter 4 MicroRNAs Reprogram Tumor Immune Response
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    Chapter 5 Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like Gene Expression, RNA Editing, and MicroRNAs Regulation
  7. Altmetric Badge
    Chapter 6 MicroRNAs Change the Landscape of Cancer Resistance
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    Chapter 7 MicroRNA, Noise, and Gene Expression Regulation
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    Chapter 8 Deep Sequencing Reveals a MicroRNA Expression Signature in Triple-Negative Breast Cancer
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    Chapter 9 Detection of Plasma MicroRNA Signature in Osteosarcoma Patients
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    Chapter 10 Identification of E6/E7-Dependent MicroRNAs in HPV-Positive Cancer Cells
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    Chapter 11 Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy
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    Chapter 12 Evaluation of MicroRNA Delivery In Vivo
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    Chapter 13 Angiogenesis Analysis by In Vitro Coculture Assays in Transwell Chambers in Ovarian Cancer
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    Chapter 14 Application of Individual qPCR Performance Parameters for Quality Control of Circulating MicroRNA Data
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    Chapter 15 Construction of Multi-Potent MicroRNA Sponge and Its Functional Evaluation
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    Chapter 16 MicroRNA Sequencing Data Analysis Toolkits
Attention for Chapter 14: Application of Individual qPCR Performance Parameters for Quality Control of Circulating MicroRNA Data
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Chapter title
Application of Individual qPCR Performance Parameters for Quality Control of Circulating MicroRNA Data
Chapter number 14
Book title
MicroRNA and Cancer
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7435-1_14
Pubmed ID
Book ISBNs
978-1-4939-7433-7, 978-1-4939-7435-1
Authors

Anna Brunet-Vega, María Elisa Quílez, María José Ramírez-Lázaro, Sergio Lario, Brunet-Vega, Anna, Quílez, María Elisa, Ramírez-Lázaro, María José, Lario, Sergio

Abstract

Although circulating miRNAs are promising candidates for biomarkers, several challenges must be overcome before miRNAs can be used for diagnosis and monitoring. One is quality control for the RNA extraction and quantification process. RNA quality control techniques are unsuitable as circulating miRNAs are in the fM range. Additionally, biofluids may contain inhibitors of the reverse transcriptase and polymerase enzymes, which may survive RNA purification. Herein, we describe the protocol we have used to check the robustness of miRNA purification and measurement by the addition of spike-ins and by evaluating the quality of the qPCR data, respectively.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 25%
Student > Ph. D. Student 1 13%
Student > Bachelor 1 13%
Other 1 13%
Unknown 3 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 25%
Medicine and Dentistry 1 13%
Unknown 5 63%