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Human Retroviruses

Overview of attention for book
Cover of 'Human Retroviruses'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay
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    Chapter 2 CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α
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    Chapter 3 The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating
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    Chapter 4 The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells
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    Chapter 5 Imaging HIV-1 Nuclear Pre-integration Complexes
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    Chapter 6 HIV-1 Reverse Transcription
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    Chapter 7 RNase H: Specificity, Mechanisms of Action, and Antiviral Target
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    Chapter 8 HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat
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    Chapter 9 HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export.
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    Chapter 10 HIV-1 Accessory Proteins: Nef
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    Chapter 11 HIV-1 Accessory Proteins: VpR
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    Chapter 12 HIV-1 Accessory Proteins: Vpu and Vif.
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    Chapter 13 SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells.
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    Chapter 14 Imaging of HIV Assembly and Release
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    Chapter 15 HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells
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    Chapter 16 Determination of HIV-1 Co-receptor Usage
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    Chapter 17 The Macrophage and HIV: Basic Concepts and Methodologies
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    Chapter 18 HIV infection of dendritic cells.
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    Chapter 19 Histocultures (Tissue Explants) in Human Retrovirology
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    Chapter 20 Single-Copy Quantification of HIV-1 in Clinical Samples.
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    Chapter 21 Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells
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    Chapter 22 HIV-1-Based Lentiviral Vectors
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    Chapter 23 Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone
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    Chapter 24 Investigating human T cell lymphotropic retrovirus (HTLV) tax function with molecular and immunophenotypic techniques.
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    Chapter 25 Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR.
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    Chapter 26 Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real-Time RT-PCR (qRT-PCR).
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    Chapter 27 Erratum To: Proviral Load Determination of HTLV-1 and HTLV-2 in Patients’ Peripheral Blood Mononuclear Cells by Real-Time PCR
Attention for Chapter 9: HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export.
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Chapter title
HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export.
Chapter number 9
Book title
Human Retroviruses
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-670-2_9
Pubmed ID
Book ISBNs
978-1-62703-669-6, 978-1-62703-670-2
Authors

Alan Cochrane, Cochrane, Alan

Abstract

The requirement of HIV-1 and HTLV-1 to export incompletely spliced mRNAs has necessitated the evolution of Rev and Rex, respectively, to overcome host cellular mechanism that block nuclear-cytoplasmic export of incompletely processed mRNAs. Evaluating the function of these viral factors can be done at multiple levels: examining the functional consequence of Rev/Rex on viral gene expression, monitoring the movement of these proteins between the nucleus and cytoplasm or the subcellular distribution of the viral mRNAs. Here, I describe procedures to evaluate each of these aspects of Rev/Rex function.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 7%
Unknown 13 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 21%
Student > Ph. D. Student 3 21%
Student > Bachelor 2 14%
Other 1 7%
Professor 1 7%
Other 2 14%
Unknown 2 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 29%
Agricultural and Biological Sciences 4 29%
Immunology and Microbiology 3 21%
Neuroscience 1 7%
Unknown 2 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2013.
All research outputs
#18,308,895
of 22,668,244 outputs
Outputs from Methods in molecular biology
#7,828
of 13,037 outputs
Outputs of similar age
#229,091
of 304,977 outputs
Outputs of similar age from Methods in molecular biology
#293
of 594 outputs
Altmetric has tracked 22,668,244 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,037 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 304,977 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 594 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.