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Mendeley readers
| Chapter title |
Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation
|
|---|---|
| Chapter number | 2 |
| Book title |
Cyclin-Dependent Kinase (CDK) Inhibitors
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2926-9_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2925-2, 978-1-4939-2926-9
|
| Authors |
Edurne Gallastegui, Oriol Bachs |
| Abstract |
Cyclin-dependent kinases (Cdks) belong to a family of key regulators of cell division cycle and transcription. Their activity is mainly regulated by association with regulatory subunits named cyclins but their activities are also regulated by phosphorylation, acetylation, and the association with specific inhibitory proteins (CKIs). The activity of different Cdks is deregulated in many different type of tumors, and thus, Cdks are considered targets for antitumoral therapy. For large screenings of inhibitors the use of purified recombinant Cdks and cyclins is recommended. We report here the current methods to determine their in vitro activity for large screenings of inhibitors. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 33% |
| Student > Ph. D. Student | 1 | 33% |
| Unknown | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 33% |
| Biochemistry, Genetics and Molecular Biology | 1 | 33% |
| Unknown | 1 | 33% |