↓ Skip to main content

Cyclin-Dependent Kinase (CDK) Inhibitors

Overview of attention for book
Cover of 'Cyclin-Dependent Kinase (CDK) Inhibitors'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Cyclin-Dependent Kinase (CDK) Inhibitors
  3. Altmetric Badge
    Chapter 2 Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation
  4. Altmetric Badge
    Chapter 3 Expression and Purification of Recombinant CDKs: CDK7, CDK8, and CDK9
  5. Altmetric Badge
    Chapter 4 Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination
  6. Altmetric Badge
    Chapter 5 Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors
  7. Altmetric Badge
    Chapter 6 Protein-Protein Interaction for the De Novo Design of Cyclin-Dependent Kinase Peptide Inhibitors
  8. Altmetric Badge
    Chapter 7 Identification of Cyclin A Binders with a Fluorescent Peptide Sensor
  9. Altmetric Badge
    Chapter 8 Cyclin-Dependent Kinase (CDK) Inhibitors
  10. Altmetric Badge
    Chapter 9 Analysis of CDK Inhibitor Action on Mitochondria-Mediated Apoptosis
  11. Altmetric Badge
    Chapter 10 Evaluating the Effects of CDK Inhibitors in Ischemia–Reperfusion Injury Models
  12. Altmetric Badge
    Chapter 11 Assessing Cell Cycle Independent Function of the CDK Inhibitor p21(CDKN1A) in DNA Repair.
  13. Altmetric Badge
    Chapter 12 Drug Delivery Strategies of Chemical CDK Inhibitors
  14. Altmetric Badge
    Chapter 13 Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs.
  15. Altmetric Badge
    Chapter 14 Evaluating Chemical CDK Inhibitors as Cell Death Inducers
  16. Altmetric Badge
    Chapter 15 Models for the Study of the Cross Talk Between Inflammation and Cell Cycle
  17. Altmetric Badge
    Chapter 16 Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors
Attention for Chapter 11: Assessing Cell Cycle Independent Function of the CDK Inhibitor p21(CDKN1A) in DNA Repair.
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
7 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Assessing Cell Cycle Independent Function of the CDK Inhibitor p21(CDKN1A) in DNA Repair.
Chapter number 11
Book title
Cyclin-Dependent Kinase (CDK) Inhibitors
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-2926-9_11
Pubmed ID
Book ISBNs
978-1-4939-2925-2, 978-1-4939-2926-9
Authors

Dutto, Ilaria, Tillhon, Micol, Prosperi, Ennio, Ilaria Dutto, Micol Tillhon, Ennio Prosperi

Abstract

The cyclin-dependent kinase (CDK) inhibitor p21(CDKN1A) is a small protein that is able to regulate many important cell functions, often independently of its activity of CDK inhibitor. In addition to cell cycle, this protein regulates cell transcription, apoptosis, cell motility, and DNA repair. In particular, p21 may participate in different DNA repair processes, like the nucleotide excision repair (NER), base excision repair (BER), and double-strand breaks (DSB) repair, because of its ability to interact with DNA repair proteins, such as proliferating cell nuclear antigen (PCNA), a master regulator of many DNA transactions. Although this role has been debated for a long time, the influence of p21 in DNA repair has been now established. However, it remain to be clarified how this role is coupled to proteasomal degradation that has been shown to occur after DNA damage. This chapter describes procedures to study p21 protein recruitment to localized DNA damage sites in the cell nucleus. In particular, we describe a technique based on local irrradiation with UV light through a polycarbonate filter with micropores; an in situ lysis procedure to detect chromatin-bound proteins by immunofluorescence; a cell fractionation procedure to study chromatin association of p21 by Western blot analysis, and p21 protein-protein interactions by an immunoprecipitation assay.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 2 29%
Unknown 5 71%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 43%
Student > Bachelor 2 29%
Unknown 2 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Medicine and Dentistry 2 29%
Unknown 3 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2015.
All research outputs
#20,284,384
of 22,818,766 outputs
Outputs from Methods in molecular biology
#9,912
of 13,121 outputs
Outputs of similar age
#330,559
of 393,507 outputs
Outputs of similar age from Methods in molecular biology
#1,053
of 1,470 outputs
Altmetric has tracked 22,818,766 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,121 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,507 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.