↓ Skip to main content

Preface.

Overview of attention for book
Cover of 'Preface.'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 RNA-Protein Complexes and Interactions
  3. Altmetric Badge
    Chapter 2 Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)
  4. Altmetric Badge
    Chapter 3 Biotin–Streptavidin Affinity Purification of RNA–Protein Complexes Assembled In Vitro
  5. Altmetric Badge
    Chapter 4 Detecting RNA–Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting
  6. Altmetric Badge
    Chapter 5 Purification of RNA–Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture
  7. Altmetric Badge
    Chapter 6 Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts
  8. Altmetric Badge
    Chapter 7 RNA-Protein Complexes and Interactions
  9. Altmetric Badge
    Chapter 8 Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases
  10. Altmetric Badge
    Chapter 9 RNA-Protein Complexes and Interactions
  11. Altmetric Badge
    Chapter 10 Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixture
  12. Altmetric Badge
    Chapter 11 RNA-Protein Complexes and Interactions
  13. Altmetric Badge
    Chapter 12 RNA-Protein Complexes and Interactions
  14. Altmetric Badge
    Chapter 13 RNA-Protein Complexes and Interactions
  15. Altmetric Badge
    Chapter 14 Ribo-Proteomics Approach to Profile RNA–Protein and Protein–Protein Interaction Networks
  16. Altmetric Badge
    Chapter 15 RNA-Protein Complexes and Interactions
  17. Altmetric Badge
    Chapter 16 Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
  18. Altmetric Badge
    Chapter 17 RNA-Protein Complexes and Interactions
  19. Altmetric Badge
    Chapter 18 RNA-Protein Complexes and Interactions
  20. Altmetric Badge
    Chapter 19 Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5′ Splice-Site Base-Pairing
  21. Altmetric Badge
    Chapter 20 Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter
Attention for Chapter 6: Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts
Altmetric Badge

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
7 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts
Chapter number 6
Book title
RNA-Protein Complexes and Interactions
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3591-8_6
Pubmed ID
Book ISBNs
978-1-4939-3589-5, 978-1-4939-3591-8
Authors

Keith T. Gagnon, Gagnon, Keith T.

Abstract

Argonaute (Ago) proteins are the minimum core proteins required for executing RNA interference (RNAi) mechanisms of gene regulation. For Ago proteins to regulate gene expression through RNAi they must be loaded, or "programmed," with a single strand of small RNA. Natural small RNAs are typically double-stranded duplexes that require additional factors for efficient and specific loading into Ago proteins. Here, a protocol is described for investigating RNAi programming through loading of human Ago2 using radiolabeled small interfering RNA (siRNA) and HeLa cell extracts. This protocol provides an Ago loading assay to study RNAi programming when starting with crude or partially purified cell extracts. The Ago loading assay should prove useful for studying other Ago proteins using a variety of mammalian cell extracts.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 43%
Professor > Associate Professor 1 14%
Student > Postgraduate 1 14%
Unknown 2 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 43%
Decision Sciences 1 14%
Neuroscience 1 14%
Unknown 2 29%