Chapter 1 History of the molecular biology of cytomegaloviruses.

Chapter 2 Overview of Human Cytomegalovirus Pathogenesis

Chapter 3 Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies

Chapter 4 Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection

Chapter 5 Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants

Chapter 6 The use of primary human cells (fibroblasts, monocytes, and others) to assess human cytomegalovirus function.

Chapter 7 Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.

Chapter 8 Analysis of Cytomegalovirus Binding/Entry-Mediated Events

Chapter 9 Use of 5-Ethynyl-2′-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression

Chapter 10 Methods for Studying the Function of Cytomegalovirus GPCRs

Chapter 11 Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues

Chapter 12 Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression

Chapter 13 Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs

Chapter 14 Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.

Chapter 15 What We Have Learned from Animal Models of HCMV

Chapter 17 Recent approaches and strategies in the generation of antihuman cytomegalovirus vaccines.

Chapter 18 Approaches for the Generation of New Anti-cytomegalovirus Agents: Identification of Protein–Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein