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Human Cytomegaloviruses

Overview of attention for book
Cover of 'Human Cytomegaloviruses'

Table of Contents

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    Book Overview
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    Chapter 1 History of the molecular biology of cytomegaloviruses.
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    Chapter 2 Overview of Human Cytomegalovirus Pathogenesis
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    Chapter 3 Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies
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    Chapter 4 Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection
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    Chapter 5 Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants
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    Chapter 6 The use of primary human cells (fibroblasts, monocytes, and others) to assess human cytomegalovirus function.
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    Chapter 7 Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.
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    Chapter 8 Analysis of Cytomegalovirus Binding/Entry-Mediated Events
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    Chapter 9 Use of 5-Ethynyl-2′-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression
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    Chapter 10 Methods for Studying the Function of Cytomegalovirus GPCRs
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    Chapter 11 Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues
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    Chapter 12 Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression
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    Chapter 13 Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs
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    Chapter 14 Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.
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    Chapter 15 What We Have Learned from Animal Models of HCMV
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    Chapter 16 Rodent models of congenital cytomegalovirus infection.
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    Chapter 17 Recent approaches and strategies in the generation of antihuman cytomegalovirus vaccines.
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    Chapter 18 Approaches for the Generation of New Anti-cytomegalovirus Agents: Identification of Protein–Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein
Attention for Chapter 7: Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.
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  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

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Chapter title
Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.
Chapter number 7
Book title
Human Cytomegaloviruses
Published in
Methods in molecular biology, March 2014
DOI 10.1007/978-1-62703-788-4_7
Pubmed ID
Book ISBNs
978-1-62703-787-7, 978-1-62703-788-4
Authors

Umashankar M, Goodrum F, Mahadevaiah Umashankar, Felicia Goodrum

Abstract

Of the many research challenges posed by human cytomegalovirus latency, perhaps the most notable is the requirement for primary hematopoietic cell culture. Culturing hematopoietic subpopulations while maintaining physiological relevance must be given utmost consideration. We describe a long-standing primary CD34(+) hematopoietic progenitor cell (HPCs) system as an experimental model to study human cytomegalovirus (HCMV) latency and reactivation. Key aspects of our model include infection of primary human CD34(+) HPCs prior to ex vivo expansion, maintenance of undifferentiated cells in a long-term culture with a stromal cell support, and an assay to quantitate infectious centers produced prior to and following a reactivation stimulus. Our method offers a unique way to quantitatively assess HCMV latency and reactivation to study the contribution of viral and host genes in latency and reactivation.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 42%
Researcher 3 25%
Student > Doctoral Student 2 17%
Student > Bachelor 1 8%
Professor > Associate Professor 1 8%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 50%
Biochemistry, Genetics and Molecular Biology 2 17%
Medicine and Dentistry 2 17%
Immunology and Microbiology 1 8%
Neuroscience 1 8%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2014.
All research outputs
#2,296,948
of 4,507,652 outputs
Outputs from Methods in molecular biology
#843
of 3,194 outputs
Outputs of similar age
#62,994
of 124,769 outputs
Outputs of similar age from Methods in molecular biology
#20
of 70 outputs
Altmetric has tracked 4,507,652 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,194 research outputs from this source. They receive a mean Attention Score of 1.4. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 124,769 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 70 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.