| Chapter title |
The use of cultured Drosophila cells for studying the microtubule cytoskeleton.
|
|---|---|
| Chapter number | 6 |
| Book title |
Mitosis
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-4939-0329-0_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-0328-3, 978-1-4939-0329-0
|
| Authors |
Jonathan Nye, Daniel W Buster, Gregory C Rogers, Daniel W. Buster, Gregory C. Rogers, Nye, Jonathan, Buster, Daniel W., Rogers, Gregory C. |
| Abstract |
Cultured Drosophila cell lines have been developed into a powerful tool for studying a wide variety of cellular processes. Their ability to be easily and cheaply cultured as well as their susceptibility to protein knockdown via double-stranded RNA-mediated interference (RNAi) has made them the model system of choice for many researchers in the fields of cell biology and functional genomics. Here we describe basic techniques for gene knockdown, transgene expression, preparation for fluorescence microscopy, and centrosome enrichment using cultured Drosophila cells with an emphasis on studying the microtubule cytoskeleton. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 31% |
| Student > Doctoral Student | 2 | 15% |
| Student > Ph. D. Student | 2 | 15% |
| Student > Master | 2 | 15% |
| Other | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 46% |
| Agricultural and Biological Sciences | 5 | 38% |
| Unknown | 2 | 15% |