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Mitosis

Overview of attention for book
Cover of 'Mitosis'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of mitotic protein dynamics and function in Drosophila embryos by live cell imaging and quantitative modeling.
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    Chapter 2 Rapid measurement of mitotic spindle orientation in cultured Mammalian cells.
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    Chapter 3 Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Microcopy Images of C. elegans Embryos.
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    Chapter 4 Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells
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    Chapter 5 Mitosis
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    Chapter 6 The use of cultured Drosophila cells for studying the microtubule cytoskeleton.
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    Chapter 7 Measuring Microtubule Growth and Gliding in Caenorhabditis elegans Embryos.
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    Chapter 8 Xenopus Egg Extracts as a Simplified Model System for Structure–Function Studies of Dynein Regulators
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    Chapter 9 Covalent Immobilization of Microtubules on Glass Surfaces for Molecular Motor Force Measurements and Other Single-Molecule Assays
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    Chapter 10 An Improved Optical Tweezers Assay for Measuring the Force Generation of Single Kinesin Molecules
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    Chapter 11 Seeded Microtubule Growth for Cryoelectron Microscopy of End-Binding Proteins
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    Chapter 12 The Segmentation of Microtubules in Electron Tomograms Using Amira
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    Chapter 13 Manipulating Cell Shape by Placing Cells into Micro-fabricated Chambers
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    Chapter 14 Four-Color FISH for the Detection of Low-Level Aneuploidy in Interphase Cells
Attention for Chapter 3: Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Microcopy Images of C. elegans Embryos.
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Chapter title
Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Microcopy Images of C. elegans Embryos.
Chapter number 3
Book title
Mitosis
Published in
Methods in molecular biology, March 2014
DOI 10.1007/978-1-4939-0329-0_3
Pubmed ID
24633792
Book ISBNs
978-1-4939-0328-3, 978-1-4939-0329-0
Authors

Farhadifar R, Needleman D, Reza Farhadifar, Daniel Needleman, Farhadifar, Reza, Needleman, Daniel

Abstract

Differential interference contrast (DIC) microscopy is a non-fluorescent microscopy technique that is commonly used to visualize the first mitotic spindle in C. elegans embryos. DIC movies are easy to acquire and provide data with high spatial and temporal resolution, allowing detailed investigations of the dynamics of the spindle-which elongates, oscillates, and is positioned asymmetrically. Despite the immense amount of information such movies provide, they are normally only used to draw qualitative conclusion based on manual inspection. We have developed an algorithm to automatically segment the mitotic spindle in DIC movies of C. elegans embryos, determine the position of centrosomes, quantify the morphology and motions of the spindle, and track these features over time. This method should be widely useful for studying the first mitotic spindle in C. elegans.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 42%
Student > Ph. D. Student 2 17%
Student > Master 1 8%
Student > Doctoral Student 1 8%
Professor > Associate Professor 1 8%
Other 1 8%
Unknown 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 50%
Agricultural and Biological Sciences 4 33%
Physics and Astronomy 1 8%
Unknown 1 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 November 2014.
All research outputs
#18,381,794
of 22,768,097 outputs
Outputs from Methods in molecular biology
#7,871
of 13,090 outputs
Outputs of similar age
#162,142
of 223,401 outputs
Outputs of similar age from Methods in molecular biology
#55
of 160 outputs
Altmetric has tracked 22,768,097 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 160 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.

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