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Mitosis

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Cover of 'Mitosis'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of mitotic protein dynamics and function in Drosophila embryos by live cell imaging and quantitative modeling.
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    Chapter 2 Rapid measurement of mitotic spindle orientation in cultured Mammalian cells.
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    Chapter 3 Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Microcopy Images of C. elegans Embryos.
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    Chapter 4 Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells
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    Chapter 5 Mitosis
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    Chapter 6 The use of cultured Drosophila cells for studying the microtubule cytoskeleton.
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    Chapter 7 Measuring Microtubule Growth and Gliding in Caenorhabditis elegans Embryos.
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    Chapter 8 Xenopus Egg Extracts as a Simplified Model System for Structure–Function Studies of Dynein Regulators
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    Chapter 9 Covalent Immobilization of Microtubules on Glass Surfaces for Molecular Motor Force Measurements and Other Single-Molecule Assays
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    Chapter 10 An Improved Optical Tweezers Assay for Measuring the Force Generation of Single Kinesin Molecules
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    Chapter 11 Seeded Microtubule Growth for Cryoelectron Microscopy of End-Binding Proteins
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    Chapter 12 The Segmentation of Microtubules in Electron Tomograms Using Amira
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    Chapter 13 Manipulating Cell Shape by Placing Cells into Micro-fabricated Chambers
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    Chapter 14 Four-Color FISH for the Detection of Low-Level Aneuploidy in Interphase Cells
Attention for Chapter 7: Measuring Microtubule Growth and Gliding in Caenorhabditis elegans Embryos.
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Chapter title
Measuring Microtubule Growth and Gliding in Caenorhabditis elegans Embryos.
Chapter number 7
Book title
Mitosis
Published in
Methods in molecular biology, March 2014
DOI 10.1007/978-1-4939-0329-0_7
Pubmed ID
Book ISBNs
978-1-4939-0328-3, 978-1-4939-0329-0
Authors

Tegha-Dunghu J, Gusnowski EM, Srayko M, Justus Tegha-Dunghu, Eva M. Gusnowski, Martin Srayko, Tegha-Dunghu, Justus, Gusnowski, Eva M., Srayko, Martin

Abstract

Microtubule plus-tip tracking is a powerful method to measure microtubule growth dynamics in vivo. Here we outline an approach that exploits live confocal microscopy of a GFP-tagged EB1-like protein to measure microtubule growth behavior and minus-end-directed microtubule motor activity at the cortex of Caenorhabditis elegans embryos. The EB1 velocity assay (EVA) provides a method to reproducibly monitor motor- and non-motor-assisted microtubule movements.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 75%
Lecturer > Senior Lecturer 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Agricultural and Biological Sciences 2 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 November 2014.
All research outputs
#20,242,136
of 22,769,322 outputs
Outputs from Methods in molecular biology
#9,865
of 13,090 outputs
Outputs of similar age
#191,528
of 223,401 outputs
Outputs of similar age from Methods in molecular biology
#95
of 160 outputs
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So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 160 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.