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Macromolecular Drug Delivery

Overview of attention for book
Attention for Chapter 3: siRNA and DNA transfer to cultured cells.
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)
  • Good Attention Score compared to outputs of the same age and source (79th percentile)

Mentioned by

twitter
2 tweeters

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
23 Mendeley
citeulike
1 CiteULike
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Chapter title
siRNA and DNA transfer to cultured cells.
Chapter number 3
Book title
Macromolecular Drug Delivery
Published in
Methods in molecular biology, January 2009
DOI 10.1007/978-1-59745-429-2_3
Pubmed ID
Book ISBNs
978-1-58829-999-4, 978-1-59745-429-2
Authors

Bagavathi Gopalakrishnan, Jon Wolff

Abstract

Transfection is a powerful non-viral technology used to deliver foreign nucleic acids into eukaryotic cells, and is the method of choice for a variety of applications including studying the functional role of particular genes and the proteins they code for. By over-expressing genes to produce protein of interest and also by knocking down specific genes, researchers are able to accurately define the role of genes and the protein they encode in various cellular processes. Therefore, this powerful technology is a very vital component of the array of scientific research tools. However, the exact mechanism of action of transfection and also the numerous factors that influence the success of DNA or RNA delivery processes are not clearly understood. Hence, this chapter attempts to explain some of the popular cationic lipid/polymer-based transfection reagents for in vitro DNA/small inhibitory RNA (siRNA) delivery, mainly focusing on the protocols and critical factors to keep in mind to ensure successful delivery of nucleic acids into eukaryotic cells using these methods.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Serbia 1 4%
Norway 1 4%
Unknown 21 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 22%
Unspecified 3 13%
Student > Bachelor 3 13%
Professor 3 13%
Researcher 3 13%
Other 5 22%
Unknown 1 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 48%
Unspecified 3 13%
Medicine and Dentistry 3 13%
Physics and Astronomy 2 9%
Arts and Humanities 1 4%
Other 3 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 November 2011.
All research outputs
#1,993,917
of 4,505,992 outputs
Outputs from Methods in molecular biology
#630
of 3,194 outputs
Outputs of similar age
#23,173
of 73,578 outputs
Outputs of similar age from Methods in molecular biology
#11
of 53 outputs
Altmetric has tracked 4,505,992 research outputs across all sources so far. This one has received more attention than most of these and is in the 53rd percentile.
So far Altmetric has tracked 3,194 research outputs from this source. They receive a mean Attention Score of 1.3. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 73,578 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 53 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 79% of its contemporaries.