Chapter title |
Covalent cross-linking of kinases with their corresponding peptide substrates.
|
---|---|
Chapter number | 12 |
Book title |
Kinase Inhibitors
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-337-0_12 |
Pubmed ID | |
Book ISBNs |
978-1-61779-336-3, 978-1-61779-337-0
|
Authors |
Alexander V. Statsuk, Kevan M. Shokat, Statsuk, Alexander V., Shokat, Kevan M. |
Abstract |
Protein phosphorylation represents the most dominant and evolutionary conserved posttranslational modification for information transfer in cells and organisms. The human genome encodes >500 protein kinases, and thousands of phosphorylation sites are present in mammalian proteome. To develop a global view of phosphorylation network, there is a need to map the connectivity between kinases and phosphoproteome. We developed a chemical kinase-substrate cross-linker 1 that converts transient kinase-substrate interactions into a covalently linked kinase-substrate complex in vitro and in the presence of cell lysates. The method can be applied to identify unknown upstream kinases responsible for phosphorylation events in cell lysates. |
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Researcher | 3 | 20% |
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