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Next-Generation MicroRNA Expression Profiling Technology

Overview of attention for book
Cover of 'Next-Generation MicroRNA Expression Profiling Technology'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Controlling miRNA Regulation in Disease
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    Chapter 2 Introduction to miRNA Profiling Technologies and Cross-Platform Comparison.
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    Chapter 3 Stem-Loop RT-qPCR for MicroRNA Expression Profiling
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    Chapter 4 Poly(T) adaptor rt-PCR.
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    Chapter 5 MicroRNA In Situ Hybridization
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    Chapter 6 Agilent MicroRNA Microarray Profiling System
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    Chapter 7 miRNA Expression Profiling Using Illumina Universal BeadChips
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    Chapter 8 MicroRNA Expression Analysis Using the Affymetrix Platform.
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    Chapter 9 Individualized miRNA Assay Panels Using Optically Encoded Beads
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    Chapter 10 Microfluidic Primer Extension Assay
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    Chapter 11 MicroRNA Profiling Using µParaflo Microfluidic Array Technology.
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    Chapter 12 MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform.
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    Chapter 13 Next-Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application.
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    Chapter 14 Methods for small RNA preparation for digital gene expression profiling by next-generation sequencing.
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    Chapter 15 Profiling of Short RNAs Using Helicos Single-Molecule Sequencing.
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    Chapter 16 deepBase: Annotation and Discovery of MicroRNAs and Other Noncoding RNAs from Deep-Sequencing Data.
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    Chapter 17 PhenomiR: MicroRNAs in Human Diseases and Biological Processes
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    Chapter 18 miRNA Expression Profiling: From Reference Genes to Global Mean Normalization
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    Chapter 19 miRNA Data Analysis: Next-Gen Sequencing.
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    Chapter 20 Integrated miRNA Expression Analysis and Target Prediction.
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    Chapter 21 miRNAs in Human Cancer.
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    Chapter 22 Blood-Based miRNA Preparation for Noninvasive Biomarker Development.
Attention for Chapter 13: Next-Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application.
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Chapter title
Next-Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application.
Chapter number 13
Book title
Next-Generation MicroRNA Expression Profiling Technology
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-427-8_13
Pubmed ID
Book ISBNs
978-1-61779-426-1, 978-1-61779-427-8
Authors

Ana Raquel Soares, Patrícia M. Pereira, Manuel A. S. Santos, Soares, Ana Raquel, Pereira, Patrícia M., Santos, Manuel A. S.

Abstract

MicroRNAs (miRNAs) are a class of small RNAs (sRNAs) of approximately 22 nucleotides in length that control eukaryotic gene expression at the translational level. They regulate a wide variety of biological processes, namely developmental timing, cell differentiation, cell proliferation, the immune response, and infection. Their identification is essential to understand eukaryotic biology. Their small size, low abundance, and high instability complicated early identification, however new generation genome sequencing approaches, such as the Roche 454 Pyrosequencer, allow for both miRNA identification and for generating miRNA profiles in a given sample. This technique avoids cloning steps in bacteria and is a fast and bias-minimized tool to discover novel miRNAs and other sRNAs on a genome-wide scale. Prior to sequencing, cDNA libraries are built for each sample using total RNA as starter material. Each cDNA library can be tagged with specific identifier sequences that allow sequencing different samples in the same chip run. Here, we describe the protocols for the construction of sRNA cDNA libraries for 454 sequencing, and we include tips for overcoming problems often encountered during cDNA library preparation.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 5%
Chile 1 5%
Sweden 1 5%
Italy 1 5%
Unknown 17 81%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 33%
Student > Ph. D. Student 4 19%
Student > Master 3 14%
Professor > Associate Professor 2 10%
Student > Bachelor 1 5%
Other 2 10%
Unknown 2 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 62%
Biochemistry, Genetics and Molecular Biology 3 14%
Computer Science 1 5%
Social Sciences 1 5%
Medicine and Dentistry 1 5%
Other 0 0%
Unknown 2 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 December 2011.
All research outputs
#10,383,951
of 17,367,552 outputs
Outputs from Methods in molecular biology
#2,773
of 9,919 outputs
Outputs of similar age
#119,482
of 222,079 outputs
Outputs of similar age from Methods in molecular biology
#200
of 466 outputs
Altmetric has tracked 17,367,552 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,919 research outputs from this source. They receive a mean Attention Score of 2.7. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 222,079 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 466 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.