Chapter title |
Transcriptional Regulation in Adipogenesis Through PPARγ-Dependent and -Independent Mechanisms by Prostaglandins.
|
---|---|
Chapter number | 15 |
Book title |
Transcription Factor Regulatory Networks
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-0805-9_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0804-2, 978-1-4939-0805-9
|
Authors |
Ko Fujimori, Yoshihiro Urade, Fujimori, Ko, Urade, Yoshihiro |
Abstract |
Adipogenesis is controlled by complex mechanisms, and transcription factors are involved in its regulation. PPARγ is a ligand-dependent transcription factor and the most important one for adipogenesis. Although prostaglandin (PG) D2 metabolites have been reported as being the ligands of PPARγ, the endogenous PPARγ ligand in adipocytes remains unclear. Here, we show the methods for the general analysis of adipocyte differentiation and the protocols for promoter analysis, fluorescence EMSA, and chromatin immunoprecipitation assay for the transcriptional regulation of the SREBP-1c-activated lipocalin-type PGD synthase gene in adipocytes. Moreover, we describe that PGD2 and its metabolites are involved in the regulation of adipogenesis through PPARγ-dependent and -independent mechanisms. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 33% |
Professor > Associate Professor | 2 | 22% |
Student > Ph. D. Student | 1 | 11% |
Other | 1 | 11% |
Student > Postgraduate | 1 | 11% |
Other | 0 | 0% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 33% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 22% |
Immunology and Microbiology | 1 | 11% |
Unknown | 3 | 33% |