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Developmental Biology of the Sea Urchin and Other Marine Invertebrates

Overview of attention for book
Cover of 'Developmental Biology of the Sea Urchin and Other Marine Invertebrates'

Table of Contents

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    Book Overview
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    Chapter 1 Culturing larvae of marine invertebrates.
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    Chapter 2 Obtaining Gametes and Embryos of Ascidians
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    Chapter 3 Artificial Seawater Culture of the Gastropod Crepidula fornicata for Studies of Larval Settlement and Metamorphosis
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    Chapter 4 Trichoplax adhaerens , an Enigmatic Basal Metazoan with Potential
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    Chapter 5 Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses
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    Chapter 6 An Invertebrate Embryologist’s Guide to Routine Processing of Confocal Images
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    Chapter 7 TEM Analyses of Chaetognath Reproductive Organs
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    Chapter 8 SEM Analysis of Marine Invertebrate Gametes
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    Chapter 9 Imaging Neural Development in Embryonic and Larval Sea Urchins
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    Chapter 10 Preparation and Use of Sea Urchin Egg Homogenates
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    Chapter 11 Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian Phallusia mammillata
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    Chapter 12 Isolating Specific Embryonic Cells of the Sea Urchin by FACS
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    Chapter 13 Biotinylation of Oocyte Cell Surface Proteins of the Starfish Patiria miniata
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    Chapter 14 Perturbations to the hedgehog pathway in sea urchin embryos.
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    Chapter 15 Regulation of DNA Synthesis at the First Cell Cycle in the Sea Urchin In Vivo
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    Chapter 16 Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte Maturation in Marine Nemertean Worms
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    Chapter 17 Multicolor Labeling in Developmental Gene Regulatory Network Analysis
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    Chapter 18 A 96-Well Plate Format for Detection of Marine Zooplankton with the Sandwich Hybridization Assay
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    Chapter 19 Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and Through the Egg-to-Embryo Transition in Sea Urchins
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    Chapter 20 Tools for Sea Urchin Genomic Analysis
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    Chapter 21 Antibody Inhibition of Protein Activity in Starfish Oocytes
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    Chapter 22 Immunophotoaffinity labeling of the binding proteins for 1-methyladenine, an oocyte maturation-inducing hormone of starfish.
Attention for Chapter 14: Perturbations to the hedgehog pathway in sea urchin embryos.
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Chapter title
Perturbations to the hedgehog pathway in sea urchin embryos.
Chapter number 14
Book title
Developmental Biology of the Sea Urchin and Other Marine Invertebrates
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-62703-974-1_14
Pubmed ID
Book ISBNs
978-1-62703-973-4, 978-1-62703-974-1
Authors

Jacob F Warner, David R McClay, Warner JF, McClay DR, Warner, Jacob F., McClay, David R., Jacob F. Warner, David R. McClay

Abstract

The Hedgehog pathway has been shown to be an important developmental signaling pathway in many organisms (Ingham and McMahon. Genes Dev 15:3059-3087, 2001). Recently that work has been extended to developing echinoderm embryos (Walton et al. Dev Biol 331(1):26-37, 2009). Here we describe several methods to perturb the Hedgehog signaling pathway in the sea urchin. These include microinjection of Morpholinos and mRNA constructs as well as treatments with small molecule inhibitors. Finally we provide simple methods for assaying Hedgehog phenotypes in the sea urchin embryo.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Student > Bachelor 1 33%
Lecturer 1 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 67%
Biochemistry, Genetics and Molecular Biology 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 February 2014.
All research outputs
#15,294,762
of 22,745,803 outputs
Outputs from Methods in molecular biology
#5,313
of 13,090 outputs
Outputs of similar age
#192,039
of 311,646 outputs
Outputs of similar age from Methods in molecular biology
#208
of 659 outputs
Altmetric has tracked 22,745,803 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 311,646 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 659 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.