| Chapter title |
Analysis of affinity of dengue virus protein interaction using biacore.
|
|---|---|
| Chapter number | 17 |
| Book title |
Dengue
|
| Published in |
Methods in molecular biology, April 2014
|
| DOI | 10.1007/978-1-4939-0348-1_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-0347-4, 978-1-4939-0348-1
|
| Authors |
Yau YH, Shochat SG, Yin Hoe Yau, Susana Geifman Shochat, Yau, Yin Hoe, Shochat, Susana Geifman |
| Abstract |
Surface plasmon resonance (SPR) biosensors have become the mainstream method for biomolecular interaction analysis. It offers many advantages over conventional methods by its label-free, real-time monitoring, low sample consumption, high throughput, and remarkable sensitivity. We have examined dengue virus protein interactions in the context of antibody affinity measurement, protein-protein interaction, and in the screening of small molecule inhibitors as well as the characterization of the interactions between the small molecule binders and the relevant dengue protein. Here we describe the basic methods involved in performing SPR assays as well as in data processing and evaluation using some examples of dengue proteins. |
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Mendeley readers
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|---|---|---|
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| Unspecified | 1 | 4% |
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